Active in Situ Biomonitoring of Pesticide Pulses Using Gammarus spp. in Small Tributaries of Lake Constance

Almut Gerhardt, Margie Koster, Frank Lang, Vera Leib

Amt für Umwelt Kanton Thurgau, Frauenfeld, Switzerland.
Amt für Umwelt und Energie Kanton St. Gallen, St. Gallen, Switzerlan.
Interkantonales Labor Schaffhausen, Schaffhausen, Switzerland;.
LimCo International GmbH, Konstanz, Germany.

DOI: 10.4236/jep.2012.37069                

Abstract

Gammarids are important members of a stream’s macrozoobenthos biocoenosis and food web. Moreover, they proved to be very sensitive towards different types of pollution. GamTox^TM is a new in situ ecotoxicity test, based on survival and feeding behavior of caged gammarids for active monitoring of small streams in agricultural areas. GamTox^TM has been applied in two streams with specific pollution problems in the catchment of Lake Constance. Ten organisms were exposed in 5 replicates in flow through test tubes containing one conditioned alder leaf, placed in baskets which were attached in the stream bottom and on the banks. Each week, the number of living animals was counted, the percentage of leaf skeletized estimated in semi-quantitative classes and a new elder leaf provided. Dead organisms were removed. Simultaneously, chemical analyses of pesticides and nutrients (N-compounds, P) were performed on cumulative water samples over one week. Moreover, macrozoobenthos was collected and determined according to the IBCH method, and the SPEAR index calculated. GamTox^TM proved to be very sensitive to detect pesticides, copper as well as nutrients, both during acute pollution pulses and chronic exposures of up to 6 weeks. Survival turned out to be a more sensitive and less variable parameter than feeding. GamTox^TM is easy to perform and directly provides a measure of ecotoxicological effects of toxicant/nutrient mixtures, which cannot be predicted by biological indices based on macrozoobenthos data such as IBCH and SPEAR-index. This study was co-financed by the InterReg IV project “?kotoxikologischer Index im Bodenseeraum”, no. 227 (2011-2013) supported by the EFRE.

Keywords

Active Biomonitoring; Gammarus; Field Test; Ecotoxicology

Conflicts of Interest

The authors declare no conflicts of interest.

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