Genetic Variation in Picea mariana × P. rubens Hybrid Populations Assessed with ISSR and RAPD Markers

Ramya Narendrula, Kabwe Nkongolo

Department of Biology, Laurentian University, Sudbury, Canada.

DOI: 10.4236/ajps.2012.36088                

Abstract

Interspecific hybridization can result in significant shifts in allele frequencies. The objective of the present study was to assess the level of genetic variation in populations of P. mariana × P. rubens hybrids derived from artificial crosses. Progenies from backcross populations created through a series of controlled pollinations among P. mariana and P. rubens trees across the hybridization index were analyzed. Several Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA (RAPD) primers were used to amplify genomic DNA samples from each population. ISSR primers produced from 30% to 52% polymorphic loci. The level of polymorphism was higher with RAPD markers, ranging from 57% to 76%. Overall, the two marker systems generated similar levels of polymorphic loci for P. mariana and P. rubens populations. No significant differences were found among the P. mariana × P. rubens populations analyzed and between the hybrids and the parental populations regardless of the molecular marker used. This confirms the genetic closeness of P. mariana and P. rubens species.

Keywords

Picea mariana; Picea rubens; Interspecific Hybrids; Inter Simple Sequence Repeat; Random Amplified Polymorphic DNA

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	R. C. Lewontin and L. C. Birch, “Hybridization as a Source of Variation for Adaptation to New Environments,” Evolution, Vol. 20, No. 3, 1966, pp. 315-336. doi:10.2307/2406633
[2]	K. Schwenk, N. Brede and B. Streit, “Introduction. Extent, Processes and Evolutionary Impact of Interspecific Hybridization in Animals,” Philosophical Transactions of the Royal Society of Biological Sciences, Vol. 363, No. 1505, 2008, pp. 2805-2811. doi:10.1098/rstb.2008.0055
[3]	M. L. Arnold, “Evolution through Genetic Exchange,” Oxford University Press, Oxford, 2007. doi:10.1093/acprof:oso/9780199229031.001.0001
[4]	J. E. Major, A. Mosseler, K. H. Johnsen, O. P. Rajora, D. C. Barsi, K.-H. Kim, J.-M. Park and M. Campbell, “Reproductive Barriers and Hybridity in Two Spruces, Picea rubens and Picea mariana, Sympatric in Eastern North America,” Canadian Journal of Botany, Vol. 83, No. 2, 2005, pp. 163-175. doi:10.1139/b04-161
[5]	S. A. M. Manley, “Genecology of Hybridization in Red Spruce (Picea rubens Sarg.) and Black Spruce (Picea mariana (Mill.) BSP),” Dissertation Abstracts International, Order No. 75-24573, 1975.
[6]	A. G. Gordon, “The Taxonomy and Genetics of Picea rubens and Its Relationship to Picea mariana,” Canadian Journal of Botany, Vol. 54, No. 9, 1976, pp. 781-813. doi:10.1139/b76-084
[7]	S. A. M. Manley and F. T. Ledig, “Photosynthesis in Blackand Red Spruce and Their Hybrid Derivatives: Ecological Isolation and Hybrid Adaptive Inferiority,” Canadian Journal of Botany, Vol. 57, No. 4, 1979, pp. 305-314. doi:10.1139/b79-042
[8]	K. H. Johnsen, J. E. Major, J. Loo and D. McPhee, “Nega- tive Heterosis Not Apparent in 22-Year-Old Hybrids of Picea mariana and Picea rubens,” Canadian Journal of Botany, Vol. 76, No. 3, 1998, pp. 434-439.
[9]	K. K. Nkongolo, “RAPD Variations among Pure and Hybrid Populations of Picea mariana, P. rubens, and P. glauca (Pinaceae), and Cytogenetic Stability of Picea Hybrids: Identification of Species-Specific RAPD Markers,” Plant Systematics and Evolution, Vol. 215, No. 1-4, 1999, pp. 229-239. doi:10.1007/BF00984657
[10]	T. Nagaoka and Y. Ogihara, “Applicability of Inter-Simple Sequence Repeat Polymorphisms in Wheat for Use as DNA Markers in Comparison to RFLP and RAPD Markers,” Theoretical Applied Genetics, Vol. 94, No. 5, 1997, pp. 597-602. doi:10.1007/s001220050456
[11]	M. S. Mehes, K. K. Nkongolo and P. Michael, “Genetic Analysis of Pinus strobus and Pinus monticola Populations from Canada Using ISSR and RAPD Markers: Development of Genome-Specific SCAR Markers,” Plant Systematics and Evolution, Vol. 267, No. 1-4, 2007, pp. 47-63. doi:10.1007/s00606-007-0534-1
[12]	F. C. Yeh and T. J. B. Boyle, “Population Genetic Analysis of Codominant and Dominant Markers and Quantitative Traits,” Belgian Journal of Botany, Vol. 129, 1997, pp. 157-163.
[13]	F. C. Yeh, R. C. Yang, T. Boyle, Z. H. Ye and J. X. Mao, “POPGENE, the User-Friendly Shareware for Population Genetic Analysis,” Molecular Biology and Biotechnology Center, University of Alberta, Edmonton, 1997.
[14]	M. Nei, “Analysis of Gene Diversity in Subdivided Populations,” Proceedings of the National Academy of Sciences of USA, Vol. 70, No. 12, 1973, pp. 3321-3323. doi:10.1073/pnas.70.12.3321
[15]	K. K. Nkongolo, L. Deverno and P. Michael, “Genetic Validation and Characterization of RAPD Markers Differentiating Black and Red Spruces: Molecular Certification of Spruce Trees and Hybrids,” Plant Systematics and Evolution, Vol. 236, No. 3-4, 2003, pp. 151-163. doi:10.1007/s00606-002-0236-7
[16]	K. K. Nkongolo, P. Michael and T. Demers, “Application of ISSR, RAPD, and Cytological Markers to the Certification of Picea mariana, P. glauca, and P. engelmannii Trees, and Their Putative Hybrids,” Genome, Vol. 48, No. 2, 2005, pp. 302-311. doi:10.1139/g04-118
[17]	D. P. Fowler, Y. S. Park and A. G. Gordon, “Genetic Variation of Red Spruce in the Maritimes,” Canadian Journal of Forest Research, Vol. 18, No. 6, 1988, pp. 703-709. doi:10.1139/x88-107
[18]	M. Perron, A. G. Gordon and J. Bousquet, “Species-Specific RAPD Fingerprints for the Closely Related Picea mariana and Picea rubens,” Theoretical Applied Genetics, Vol. 91, No. 1, 1995, pp. 142-149. doi:10.1007/BF00220871
[19]	M. Tremblay and J. P. Simon, “Genetic Structure of Marginal Populations of White Spruce (Picea glauca) at Its Northern Limit of Distribution in Nouveau-Quebec,” Canadian Journal of Forest Research, Vol. 19, No. 11, 1989, pp. 1371-1379. doi:10.1139/x89-211
[20]	J. P. Jaramillo-Correa and J. Bousquet, “New Evidence from Mitochondrial DNA of a Progenitor-Derivative Species Relationship between Black Spruce and Red Spruce (Pinaceae),” American Journal of Botany, Vol. 90, No. 12, 2003, pp. 1801-1806. doi:10.3732/ajb.90.12.1801
[21]	J. P. Jaramillo-Correa and J. Bousquet, “Mitochondrial Genome Recombination in the Zone of Contact between Two Hybridizing Conifers,” Genetics, Vol. 171, No. 4, 2005, pp. 1951-1962. doi:10.1534/genetics.105.042770
[22]	D. Q. Fang and M. L. Roose, “Identification of Closely Related Citrus Cultivars with Inter-Simple Sequence Repeat Markers,” Theoretical Applied Genetics, Vol. 95, No. 3, 1997, pp. 408-417. doi:10.1007/s001220050577
[23]	S. N. Raina, V. Rani, T. Kojima, Y. Ogihara, K. P. Singh and R. M. Devarumath, “RAPD and ISSR Fingerprints as Useful Genetic Markers for Analysis of Genetic Diversity, Varietal Identification, and Phylogenetic Relationships in Peanut (Arachis hypogaea) Cultivars and Wild Species,” Genome, Vol. 44, No. 5, 2001, pp. 763-772.
[24]	J. G. Williams, A. R. Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey, “DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers,” Nucleic Acids Research, Vol. 18, No. 22, 1990, pp. 6531- 6535. doi:10.1093/nar/18.22.6531
[25]	E. Zietkiewicz, A. Rafalski and D. Labuda, “Genome Fingerprinting by Simple Sequence Repeat (SSR)—Anchored Polymerase Chain Reaction Amplification,” Genomics, Vol. 20, No. 2, 1994, pp. 176-183. doi:10.1006/geno.1994.1151
[26]	K. Semagn, A. Bjornstad and M. N. Ndjiondjop, “An Overview of Molecular Markers Methods for Plants,” African Journal of Biotechnology, Vol. 5, No. 25, 2006, pp. 2540-2568.
[27]	W. Qian, S. Ge and D. Y. Hong, “Genetic Variation within and among Populations of a Wild Rice Oryza granulata from China Detected by RAPD and ISSR Markers,” Theoretical Applied Genetics, Vol. 102, No. 2-3, 2001, pp. 440-449. doi:10.1007/s001220051665