The disease reactions of heirloom bell pepper “California Wonder” to Phytophthora capsici

Byron L. Candole, Patrick J. Conner, Cecilia McGregor, Vickie Waters, Pingsheng Ji

Horticulture Department, University of Georgia Tifton Campus, Tifton, USA.
Horticulture Department, University of Georgia, Athens, USA.
Plant Pathology Department, University of Georgia, Athens, USA.

DOI: 10.4236/as.2012.33049                

Abstract

Greenhouse studies were performed to determine the reactions of 10 “California Wonder” (Capsicum annuum) accessions to the three forms of Phytophthora blight (root rot, stem blight and foliar blight) caused by Phytophthora capsici. Differences in root rot, stem blight and foliar blight severities among accessions were significant. The accessions consistently differentiated into two groups across the three disease syndromes. Simple sequence repeat (SSR) markers showed variability both within and between accessions of California Wonder. The variability in the responses to the three forms of Phytophthora blight does not warrant its usefulness as a standard susceptible control in studies involving the Capsicum-P. capsici patho-system.

Keywords

Pepper; Phytophthora Blight; Root Rot; Stem Blight; Foliar Blight; SSR

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Votava, E.J. and Bosland, P.W. (2002) A cultivar by any other name: Genetic variability in heirloom bell pepper “California Wonder”. HortScience, 37, 1100-1102.
[2]	Barksdale, T., Papavisas, G. and Johns-ton, S. (1984) Resistance to foliar blight and crown rot of pepper caused by Phytophthora capsici. Plant Disease, 68, 506-509.
[3]	Saini, S.S. and Sharma, P.P. (1978) Inheritance of resistance to fruit rot (Phytophthora capsici Leon.) and induction of resistance in bell pepper (Capsicum annuum L.). Euphytica, 27, 721-723. doi:10.1007/BF00023707
[4]	Sy, O., Bosland, P.W. and Steiner, R. (2005) Inheritance of Phytophthora stem blight resistance as compared to Phytophthora root rot and Phytophthora foliar blight resistance in Capsicum annuum L. Journal of the American Society of Horticultural Science, 130, 75-78.
[5]	Smith, P.G. and Minges, P.W. (1951) Resistance of strains of California Wonder pepper to Phytophthora root rot. Plant Disease Reporter, 35, 509.
[6]	Kline, W.L., Wyenandt, C.A., Ward, D.L., Sudal, J.F. and Maxwell, N.L. (2011) Evaluation of six nitrogen fertility programs on marketable yield and development of skin separation in bell pepper fruit. Hort-Technology, 21, 323- 328.
[7]	Kuhajek, J.M., Jeffers, S.N., Slattery, M. and Wedge, D.E. (2003) A rapid microbioassay for discovery of novel fungicides for Phytophthora spp. Phytopathology, 93, 46-53. doi:10.1094/PHYTO.2003.93.1.46
[8]	Bosland, P.W. and Lindsey, D.L. (1991) A seedling screen for Phytophthora root rot of pepper, Capsicum annuum. Plant Disease, 75, 1048-1050. doi:10.1094/PD-75-1048
[9]	Alcantara, T.P. and Bosland, P.W. (1994) An inexpensive disease screening technique for foliar blight of chile pepper seedlings. HortScience, 29, 1182-1183.
[10]	Kim, Y.J., Hwang, B.K. and Park, K.W. (1989) Expression of age-related resistance in pepper plants infected with Phytophthora capsici. Plant Disease, 73, 745-747. doi:10.1094/PD-73-0745
[11]	Lee, J.M., Nahm, S.H., Kim, Y.M. and Kim, B.D. (2004) Characterization and molecular genetic mapping of microsatellite loci in pepper. Theoretical and Applied Genetics, 108, 619-627. doi:10.1007/s00122-003-1467-x
[12]	Minamiyama, Y., Tsuro, M., Kubo, T. and Hirai, M. (2007) QTL analysis for resistance to Phytophthora capsici in pepper using a high density SSR-based map. Breeding Science, 57, 129-134. doi:10.1270/jsbbs.57.129
[13]	Nagy, I., Stágel, A., Sasvári, Z., R?der, M. and Ganal, M. (2007) Development, characterization, and transferability to other Solanaceae of microsatellite markers in pepper (Capsicum annuum L.). Genome, 50, 668-688. doi:10.1139/G07-047
[14]	McGregor, C., Waters, V., Nambeesan, S., MacLean, D., Candole, B.L. and Conner P. (2011) Genotypic and phenotypic variation among pepper accessions resistant to Phytophthora capsici. HortScience, 46, 1235-1240.
[15]	Liu, K. and Muse, S.V. (2005) PowerMarker: An integrated analysis environment for genetic marker analysis. Bioinformatics, 21, 2128-2129. doi:10.1093/bioinformatics/bti282
[16]	Nei, M., Tajima, F. and Tateno, Y. (1983) Accuracy of estimated phylogenetic trees from molecular data. Journal of Molecular Evolution, 19, 153-170. doi:10.1007/BF02300753
[17]	Sneath, P.H.A. and Sokal, R.R. (1973) Numerical taxonomy. W. H. Freeman, San Francisco.
[18]	Page, R.D.M. (1996) Tree view: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences: CABIOS, 12, 357-358.
[19]	Weir, B.S. (1996) Genetic data analysis II. Sinauer Associates, Inc., Sunderland.
[20]	Botstein, D., White, R.L., Skolnick, M. and Davis, R.W. (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32, 314-331.
[21]	Tommasini, L., Batley, J., Arnold, G.M., Cooke, R.J., Donini, P., Lee, D., Law, J.R., Lowe, C., Moule, C., Trick, M. and Edwards, K.J. (2003) The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties. Theoretical and Applied Genetics, 106, 1091-1101.
[22]	Shah, D.A. and Madden, L.V. (2004) Nonparametric analysis of ordinal data in designed factorial experiments. Phyto-pathology, 94, 33-43. doi:10.1094/PHYTO.2004.94.1.33
[23]	Brunner, E.S., Domhof, S. and Langer, F. (2002) Nonparametric analysis of longitudinal data in factorial experiments. John Wiley & Sons, Inc., New York.
[24]	Whealy, K. (2004) Garden seed inventory: Inventory of seed catalogs listing of all non-hybrid vegetable seeds available in the United States. 6th Edition, Seed Savers, Inc., Iowa.
[25]	Pickersgill, B. (1997) Genetic resources and breeding of Capsicum spp. Euphytica, 96, 129-133. doi:10.1023/A:1002913228101