SSINCC: Simple separation of interacting nucleoprotein complex components

Roderick A. Slavcev, Nafiseh Nafissi, Tranum Kaur

School of Pharmacy, University of Waterloo, Waterloo, Canada.

DOI: 10.4236/abc.2012.22017                

Abstract

Protein-DNA binding assays have been used in a va-riety of applications from fundamental studies re-garding the binding process itself to serve as probes for the detection, quantification and separation of target analytes. Here we describe a novel method of analyzing and identifying intermolecular DNA interactions that allows for the simple separation of interacting nucleoprotein complex components (SSINCC), focusing specifically on DNA-DNA interactions using P1 plasmid active partition system nucleoprotein complexes as a model to demonstrate DNA sequence specificity and tolerance of composite factor complexity. Traditional and recent assays of protein-DNA interaction are summarized and compared with SSINC. Although SSINC is examined here employing P1 partition nucleoprotein complex as an example of DNA-DNA intermolecular association, universal applications of this methodology to nucleo-protein complex studies can be envisioned.

Keywords

DNA-Protein-DNA Interaction Assay; Plasmid Partition Nucleoprotein Complex; Specific Nucleoprotein Complex Separation

Conflicts of Interest

The authors declare no conflicts of interest.

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