Application of Marker Assisted Backcrossing to Introgress the Submergence Tolerance QTL SUB1 into the Vietnam Elite Rice Variety-AS996

Abstract

The result of the study contributes to enhancing and sustaining future livelihoods and food security in Vietnam vis-a-vis climate change. An innovative strategy based on marker-assisted backcrossing (MABC) was used to transform popular rice variety AS996 into the one can tolerate submergence while maintaining its original characteristics preferred by farmers and consumers. The submergence tolerance QTL SUB1 counts for up to 70% of the submergence tolerant and provides a marked improvement of submergence tolerance in all genetic backgrounds and environments tested so far. Parental diversity was carried out with 460 markers. Of which, 53 polymorphic markers were used for assessment on BC1F1, BC2F1 and BC3F1 generations. The best BC1F1 plant was P422 with highest recipient allele was 87.5%, while the additional chosen plants were P412, P428, P215 and P39 (81% - 84%). All these plants were used to develop BC2F1 generation. The six BC2F1 plants were used to develop BC3F1 and BC2F2 were the plants number P422-11 and P422-14 having 93.75% recipient alleles and P422-12, P422-3, P39-17, P39-25 having 92.25% recipient alleles. Total of 445 BC3F1 plants were confirmed the introgresion of SUB1 using ART5 and SC3. After three generations of backcrossing, application of MABC resulted in the best BC3F1 individual P422-14-177 with 100% of recipient alleles based on the number of 53 markers used with only the introgression size of SUB1 was 0.3Mb between ART5 and SC3. Phenotyping was carried out on BC3F1 and BC2F2 of the selected lines. The survival ratio of these selected lines and IR64SUB1 were the same. It convinced the successfully introgress SUB1 into AS996 rice variety. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new submergence tolerant rice variety ASS996-SUB1 to cope with climate change.

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L. Cuc, L. Huyen, P. Hien, V. Hang, N. Dam, P. Mui, V. Quang, A. Ismail and L. Ham, "Application of Marker Assisted Backcrossing to Introgress the Submergence Tolerance QTL SUB1 into the Vietnam Elite Rice Variety-AS996," American Journal of Plant Sciences, Vol. 3 No. 4, 2012, pp. 528-536. doi: 10.4236/ajps.2012.34063.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] D. O. Manzanilla, T. R. Paris, G. V. Vergara, A. M. Ismail, S. Pandey, R. V. Labios, G. T. Tatlonghari, R. D. Acda, T. T. N. Chi, K. Duoangsila, I. Siliphouthone, M. O. A. Manikmas and D. J. Mackill, “Submergence Risks and Farmers’ Preferences: Implications for Breeding Sub1 Rice in Southeast Asia,” Agricultural Systems, Vol. 104, No. 4, 2011, pp. 353-347. doi:10.1016/j.agsy.2010.12.005
[2] D. J. Mackill, “Breeding for Resistance to Abiotic Stresses in Rice: The Value of Quantitative Trait Loci,” In: K. R. Lamkey and M. Lee, Eds., Plant Breeding: The Arnel R Hallauer International Symposium, Blackwell.
[3] B. C. Y. Collard and D. J. Mackill, “Marker-Assisted Selection: An Approach for Precision Plant Breeding in the 21st Century,” Philosophical Transactions of the Royal Society, Vol. 363, No. 1491, 2008, pp. 557-572. doi:10.1098/rstb.2007.2170
[4] F. Hospital, “Size of Donor Chromosome Segments around Introgressed Loci and Reduction of Linkage Drag in Markerassisted Backcross Programs,” Genetics, Vol. 158, No. 3, 2001, pp. 1363-1379.
[5] F. Hospital and A. Charcosset, “Marker-Assisted Introgression of Quantitative Trait Loci,” Genetics, Vol. 147, No. 3, 1997, pp. 1469-1485.
[6] S. Chen, X. H. Lin, C. G. Xu and Q. F. Zhang, “Improvement of Bacterial Blight Resistance of ‘Minghui 63’, an Eliteestorer Line of Hybrid Rice, by Molecular Marker-Assisted Selection,” Crop Science, Vol. 40, No. 1, 2000, pp. 239-244. doi:10.2135/cropsci2000.401239x
[7] C. N. Neeraja, R. Maghirang-Rodriguez, A. Pamplona, S. Heuer, B. C. Y. Collard, E. M. Septiningsih, G. Vergara, D. Sanchez, K. Xu, A. M. Ismail and D. J. Mackill, “A Marker-Assisted Backcross Approach for Developing Submergence-Tolerant Rice Cultivars,” Theoretical and Applied Genetics, Vol. 115, No. 6, 2007, pp. 767-776. doi:10.1007/s00122-007-0607-0
[8] E. M. Septiningsih, A. M. Pamplona, D. L. Sanchez, C. N. Neeraja, G. V. Vergara, S. Heuer, A. M. Ismail and D. J. Mackill, “Development of Submergence Tolerant Rice Cultivars: The Sub1 Locus and Beyond,” Annals of Botany, Vol. 103, No. 2, 2009, pp. 151-160. doi:10.1093/aob/mcn206
[9] K. Xu and D. J. Mackill, “A Major Locus for Submergence Tolerance Mapped on Rice Chromosome 9,” Molecular Breeding, Vol. 2, No. 3, 1996, pp. 219-224. doi:10.1007/BF00564199
[10] K. Xu, X. Xia, T. Fukao, P. Canlas, R. Maghirang-Rodriguez, S. Heuer, A. M. Ismail, J. Bailey-Serres, P. C. Ronald, D. J. Mackill, “Sub1A Is an Ethylene Response Factor-Like Gene that Confers Submergence Tolerance to Rice,” Nature, Vol. 442, No. 7103, 2006, pp. 705-708. doi:10.1038/nature04920
[11] N. T. Lang , T. T. Luy, T. T. Binh, T. D. Thuan, T. Van Canh, N. Van Bang, R. Labios, B. C. Buu, A. Ismail and D. Mackill, “Evaluation Methodology and Utilization Submergence Gene in Southern of Vietnam,” Omonrice, Vol. 17, 2010, pp. 41-53.
[12] R. Van Berloo, “GGT 2.0: Versatile Software for Visualization and Analysis of Genetic Data,” Journal of Heredity, Vol. 99, No. 2, 2008, pp. 232-236. doi:10.1093/jhered/esm109
[13] K. Xu, X. Xu, P. C. Ronald and D. J. Mackill, “A High-Resolution Linkage Map in the Vicinity of the Rice Submergence Tolerance Locus Sub1,” Molecular and General Genetics, Vol. 263, No. 4, 2000, pp. 681-689. doi:10.1007/s004380051217
[14] M. J. Thomson, M. D. Ocampo, J. Egdane, M. A. Rahman, A. G. Sajise, D. L. Adorada, E. Tumimbang-Raiz, E. Blumwald, Z. I. Seraj, R. K. Singh, G. B. Gregorio and A. M. Ismail, “Characterizing the Saltol Quantitative Trait Locus for Salinity Tolerance in Rice,” Rice, Vol. 3, No. 2-3, 2010, pp. 148-160. doi:10.1007/s12284-010-9053-8

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