Fabrication of Poly(Aspartic Acid)-Nanogold Modified Electrode and Its Application for Simultaneous Determination of Dopamine, Ascorbic Acid, and Uric Acid

Juan Li, Xiaoli Zhang

School of Chemistry and Chemical Engineering, Shandong University, Jinan, China.

DOI: 10.4236/ajac.2012.33028                

Abstract

A nanostructured polymer film incorporated gold nanoparticles modified electrode was fabricated. The fabrication process involved a previous electropolymerization of aspartic acid and followed by the eletrodeposition of gold nano-particles on the glassy carbon electrode. The resulting poly (aspartic acid)-nanogold modified electrode (PAA- nano-Au/GCE) was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectros-copy (EIS). A higher catalytic activity was obtained to electrocatalytic oxidation of dopamine (DA), ascorbic acid (AA) and uric acid (UA) due to the enhanced peak current and well-defined peak separations compared with three, bare GCE, PAA/GCE and nano-Au/GCE. Simultaneous determination of DA, AA, and UA were studied by voltammetry. The linear range of 5.0 × 10^-7 - 1.0 × 10^-4 M for DA, 5.0 × 10^-6 - 2.0 × 10^-3 M for AA and 5.0 × 10^-6 - 1.0 × 10^-3 M for UA was obtained. The detection limit was calculated for DA, AA and UA as being 6.5 × 10^-8 M, 5.6 × 10^-7 M and 3.0 × 10^-7 M, respectively (S/N = 3). The practical application of the present modified electrode was demonstrated by the determination of DA, AA and UA in calf serum and fetal calf serum samples.

Keywords

Simultaneous Determination; Poly (Aspartic Acid)-Nanogold Modified Electrode; Dopamine; Ascorbic Acid; Uric Acid; Voltammetry

Conflicts of Interest

The authors declare no conflicts of interest.

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