Share This Article:

DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead

Abstract Full-Text HTML Download Download as PDF (Size:409KB) PP. 292-297
DOI: 10.4236/ns.2010.24037    5,657 Downloads   10,917 Views   Citations

ABSTRACT

We measured in a comet assay the damage of DNA in the hemocytes of various stages of the grasshopper Schistocerca gregaria after ex-posing them to various doses of Cd and Pb in the food. The mechanisms of Cd and Pb toxicity for grasshopper are discussed. The accumula-tion of heavy metals and stage of the insect may play important roles in causing the DNA damage. S. gregaria may be considered a valuable bio-indicator for evaluation the genotoxicity of en-vironmental pollutants.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Yousef, H. , Afify, A. , Hasan, H. and Meguid, A. (2010) DNA damage in hemocytes of Schistocerca gregaria (Orthoptera: Acrididae) exposed to contaminated food with cadmium and lead. Natural Science, 2, 292-297. doi: 10.4236/ns.2010.24037.

References

[1] Michailova, P., Petrova, N., Bovero, S., Cavicchioli, O., Ramella, L. and Sella, G. (2000a) Effect of environ-mental pollution on the chromosome variability of Chi-ronomus riparius Meigen (1804) (Diptera, Chironomidae) larvae from two Piedmont stations. Genetica, 108(2), 171-180.
[2] Michailova, P., Petrova, N., Bovero, S., Sella, G. and Ramella, L. (2000b) Structural and functional rear-rangements in polytene chromosomes of Chironomids (Diptera) as biomarkers for heavy metal pollution in aquatic ecosystems. International Conference on Heavy Metals in the Environment, Michigan University, Michi-gan, 70-79.
[3] Michailova, P., Ilkova, J., Petrova, N. and White, K. (2001) Rearrangements in the salivary gland chromo-somes of Chironomus riparius Mg. (Diptera, Chironomi-dae) following exposure to lead. Caryologia, 4, 349-363.
[4] Barsiene, J. (1994) Chromosome set changes in molluscs from highly polluted habitats in: A.R. beaumont, editors, genetics and evolution of aquatic organisms. Chapman & Hall, London, 434-447.
[5] Barsyte, D. (1999) The analysis of genotoxic damage of industrial pollution in freshwater mollusc tissues. Sum-mery Doctoral Thesis, Institute of Ecology, Vilnius, 1-20.
[6] Warchalowska–Sliwa, E., Niklinska, M., Görlich, A., Michailova, P. and Pyza, E. (2005) Heavy metal accu-mulation, heat shock protein expression and cytogenetic changes in Tetrix tenuicornis (L.) (Tetrigidae, Orthoptera) from polluted areas. Environmental Pollution, 133(2), 373-381.
[7] Goering, P.L., Waalkes, M.P. and Klaassen, C.D. (1995) Toxicology of cadmium. Goyer, R.A. and Cherian, M.G., Ed., Toxicology of Metals, Springer-Verlag, Berlin, 189- 214.
[8] Sanita di Toppi, L. and Gabbrieli, R. (1999) Responses to cadmium in higher plants. Environmental and Experi-mental Botany, 41(2), 105-130.
[9] Panda, B.B. and Panda, K.K. (2002) Genotoxicity and mutagenicity of metals in plants. Prasad, K.N.V. and Strzalka, K., Ed., Physiology and BioChemistry of Metal Toxicity and Tolerance in Plants, Kluwer Academic Pub-lishers, Netherlands, 15, 395-414.
[10] Waalkes, M. and Misra, R. (1996) Cadmium carcino-genicity and genotoxicity. Chang, L.W., Ed., Toxicology of Metals, CRC Press, Boca Raton, 231-241.
[11] Arcadio, P.S. and Gregoria, A.S. (2002) Physical-chemical treatment of water and waste water. CRC Press, Boca Raton, IWA Publishing, London.
[12] Rojas, E., Lopez, M.C. and Valverde, M. (1999) Single cell gel electrophoresis assay: Methodology and applica-tions. Journal of Chromatography B: Biomedical Sci-ences and Applications, 722(1-2), 225-254.
[13] Woźniak, K. and Blasiak, J. (2003) In vitro genotoxicity of lead acetate: Induction of single and double DNA strand breaks and DNA-protein cross-links. Mutation Research, 535(2), 127-139.
[14] Siddique, H.R., Chowdhuri, D.K., Saxena, D.K. and Dhavan, A. (2005) Validation of Drosophila melan- ogaster as an in vivo model for genotoxicity assessment using modified alkaline comet assay. Mutagenesis, 20(4), 285-290.
[15] Todoriki, S., Hasan, M., Miyanoshita, A., Imamura, T. and Hayashi, T. (2006) Assessment of electron beam- induced DNA damage in larvae of chestnut weevil. Cur-culio sikkimensis (Coleoptera: Curculionidae) using comet assay. Radiation Physics and Chemistry, 75(2), 292-296.
[16] Augustyniak, M., Juchimiuk, J., Przybylowicz, W.J., Mesjasz-Przybylowicz, J., Babczynska, A. and Migula, P. (2006) Zinc-induced DNA damage and the distribution of metals in the brain of grasshoppers by the comet assay and micro-PIXE. Comparative Biochemistry and Physi-ology - Part C, 144(3), 242-251.
[17] Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1998) A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175(1), 184-191.
[18] Grover, S.A., Coupal, L., Zowall, H., Alexander, C.M., Weiss, T.W. and Gomes, D.R.J. (2001) How cost-effec-tive is the treatment of dyslipidemia in patients with dia-betes but without cardiovascular disease? Diabetes Care, 24(1), 45-50.
[19] Mourón, S.A., Golijow, C.D. and Dulout, F.N. (2001) DNA damage by cadmium and arsenic salts assessed by the single cell gel electrophoresis assay. Mutation Re-search, 498(1-2), 47-55.
[20] Tice, R., Agurell, E., Anderson, D., Burlinson, B., Hart-mann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J.C. and Sasaki, Y. (2000) Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis, 35(3), 206-221.
[21] Devkota, B. and Schmidt, G.H. (2000) Accumulation of heavy metals in food plants and grasshoppers from the Taigetos Mountains. Greece Agriculture, Ecosystems and Environment, 78(1), 85-91.
[22] Hossain, Z. and Huq, F. (2002) Studies on the interaction between Cd(2+) ions and nucleobases and nucleotides. Journal of Inorganic Biochemistry, 90(3-4), 97-105.
[23] Misra, R., Smith, G. and Waalkes, M. (1998) Evaluation of the direct genotoxic potential of cadimium in four dif-ferent rodent cell lines. Toxicology, 126(2), 103-114.
[24] Dally, H. and Hartwig, A. (1997) Induction and repair inhibition of oxidative DNA damage by nickel (II) and cadmium (II) in mammalian cells. Carcinogenesis, 18, 1021-1026.
[25] Beyersmann, D. and Hechtenberg, S. (1997) Cadmium, gene regulation, and cellular signaling in mammalian cells. Toxicology and Applied Pharmacology, 144(2), 247-261.
[26] Waalkes, M., Fox, D., States, C., Patierno, S. and McCabe, M. (2000) Metals and disorder of cell accumulation: Modulation of apoptosis and cell proliferation. Toxico-logical Sciences, 56(2), 255-261.
[27] Korsloot, A., van Gestel, C.A.M. and van Straalen, N.M. (2004) Environmental stress and cellular response in ar-thropods. CRC Press, Boca Raton.
[28] Hamer, D.H. (1986) Metallothionein. Annual Review of Biochemistry, 55, 931-951.
[29] Maroni, G.J., Wise, J., Young, J.E. and Otto, E. (1987) Metallothionein gene duplications and metal tolerance in natural populations of Drosophila melanogaster. Genetics, 117(4), 739-744.
[30] Barltop, D. and Meek, F. (1987) Effect of particle size on lead absorption from the gut. Archives of Environmental Health, 34(4), 280-286.
[31] Peng, T., Gitelman C.H. and Garner, S.C. (1979) Acute lead induced increase in serum calcium in the rat without increased secreation of calcitonin. Proceedings of the So-ciety for Experimental Biology and Medicine, 160(1), 114-117.
[32] Johnson, L. and Pellicciari, C.E. (1988) Lead induced changes in the stabilization of the mouse sperm chroma-tin. Toxicology, 51(1), 11-24.
[33] Ariza, M.E., Bijur, G.N. and Williams, M.V. (1998) Lead and mercury mutagenesis: Role of H2O2 superoxide dis-mutase and xanthine oxidase. Environmental and Mo-lecular Mutagenesis, 31(4), 352-361.
[34] Miadoková, E., Vlčková, V., Jendraššaková, N., Vlček, D. and Šucha, V. (2000) Mutagenic and comutagenic effects of acid-mine water containing heavy metals. Journal of Trace and Microprobe Techniques, 18, 201-207.
[35] Duydu, Y., Suzen, H.S., Aydin, A., Cander, O., Uysal, H., Isimer, A. and Vural, N. (2001) Correlation between lead exposure indicators and sister chromatid exchange (SCE) frequencies in lymphocytes from inorganic lead exposed workers. Archives of Environmental Contamination and Toxicology, 41(2), 241-246.

  
comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.