ABSTRACT
The objective of this study was to determine the prevalence of HPV-HR genotypes
in the population of sexually active women in Bobo-Dioulasso, Burkina Faso. Methods: This study took place at Souro Sanou Teaching Hospital in Bobo-Dioulasso
from September to June 2017. A total of 234 women in the gynecological period
and also sexually active were enrolled after they gave an individual consent.
Swabbing of the endocervical canal was done. From the sample stored at -20°C,
the viral DNA was extracted using the “DNA-Sorb-A” kit from SACACE
biotechnologies®. Amplification of the PCR of the extracted DNA was made, using
the “HPV Genotypes 14 Real-TM Quant” V67-100 FRT kit. Data were analyzed with
SPSS software version 17.0 and Epi Info 6. Chi-square and Fisher’s tests were
used to compare proportions and averages; a link was significant when p < 0.05. Results: The mean age was 30.7 ± 7.3 years (median: 30 years); 84.5% of them were
married, 43.5% had a socio-professional activity and 61% were schooled. A total of 20.6% of women
were positive for at least one of the following HPV-HR genotypes: HPV 18, 31,
35, 39, 45, 51, 52, 56, 58, 59, 66, and 68. Genotypes the most frequently found
were HPV 52 with 11 cases (17.2%) and 66 with 10 cases (15.6%). Conclusion: Our results show a predominance of HPV-HR genotypes
not covered by available vaccines. Mapping genotypes are needed to fully understand viral ecology at the national level. But for
instance, the nonavalent vaccine, which has a better coverage of the predominant
genotypes, is to be promoted.
Share and Cite:
Ouattara, S. , Somé, D. , Dembélé, A. , Sanfo, S. , Zohoncon, T. , Ouattara, A. , Bambara, M. , Dao, B. and Simporé, J. (2019) Molecular Epidemiology of High-Risk Human Papilloma Virus Infection in Sexually Active Women at Bobo-Dioulasso University Teaching Hospital.
Open Journal of Obstetrics and Gynecology,
9, 1178-1188. doi:
10.4236/ojog.2019.98114.