Isolation and Characterization of Glycopeptides with Analgesic Activities from Marasmius androsaceus

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DOI: 10.4236/ajps.2019.107085    428 Downloads   915 Views  

ABSTRACT

To screen and characterize the analgesic components from Marasmius androsaceus, the water-soluble extracts from the mycelium of M. androsaceus were isolated by ethanol precipitation followed by macroporous resin chromatography and activity-based fractionation. Analgesic activities were found to be associated with glycopeptides. The glycopeptide fractions were further purified by gel-permeation chromatography and two purified glycopeptides, D2H, D2L, were obtained. Next, the physicochemical properties and characteristics of these purified glycopeptides were studied. They were composed of glucose and mannose and 10 different amino acids and with molecular weights of 18,000 and 8000 Da, respectively. The glycosidic linkages in the purified glycopeptides were analysed via methylation analysis. The sugar portion of the glycopeptides was composed of 1 - 4 linked Glc as the main chain with side chains composed primarily of Man linked to the 6 position of the main chain; the non-reducing terminal was composed of Glc. The glycosidic band in the main chain was identified as being in the β-configuration by the IR absorbance at 891 cm-1.

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Yang, X. , Xu, D. , Gao, Q. and Gao, Y. (2019) Isolation and Characterization of Glycopeptides with Analgesic Activities from Marasmius androsaceus. American Journal of Plant Sciences, 10, 1196-1205. doi: 10.4236/ajps.2019.107085.
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