Effect of Wild Marjoram (Origanum vulgare) Plant Extracts on Capacitation of Sheep Spermatozoa in Vitro - Advances in Bioscience and Biotechnology

ABB > Vol.10 No.4, April 2019

Effect of Wild Marjoram (Origanum vulgare) Plant Extracts on Capacitation of Sheep Spermatozoa in Vitro ()

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DOI: 10.4236/abb.2019.104006 1,037 Downloads 2,487 Views Citations

Author(s)

Ebtesam S. Alenezy^1,2, Ibrahim A. H. Barakat^1,3, Nawal M. Al Musayeib⁴

Affiliation(s)

¹Zoology Department, College of Science, King Saud University, Riyadh, KSA.
²Department of Biology, College of Science, Hafr Al Batin University, Hafr Al Batin, Saudi Arabia.
³Cell Biology Department, National Research Center, Giza, Egypt.
⁴Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

ABSTRACT

The purpose of this study was to evaluate the effect of the addition of Origanum vulgare extract to in vitro capacitation sperm medium (IVCSM). This study investigated the antioxidant and antimicrobial effects of O. vulgare extracts at different concentrations (0.3, 0.6, 1.2 μg/ml and 25.0, 50.0, 100.0 μg/ml, respectively) in IVCSM. Significant enhancements in semen quality parameters such as total motility, live and live capacitated sperm were found when O. vulgare extract was added as an antioxidant source (1.2 μg/ml). The treatment of spermatozoa with O. vulgare extract at the highest concentration (100 μg/ml) for 2 hrs without antibiotics improved sperm characteristics. In conclusion, incubation of sperm with O. vulgare extract in capacitation medium had beneficial effects on the characteristics of ram sperm.

KEYWORDS

Sheep, Sperm, Origanum vulgare, In Vitro

Share and Cite:

Alenezy, E. , Barakat, I. and Al Musayeib, N. (2019) Effect of Wild Marjoram (Origanum vulgare) Plant Extracts on Capacitation of Sheep Spermatozoa in Vitro. Advances in Bioscience and Biotechnology, 10, 82-97. doi: 10.4236/abb.2019.104006.

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