ABSTRACT
Background: Hepatitis B is an infectious disease, which is a main way of vertical
transmission of infectious HBV between mother and infant. Hepatitis B virus
infection is always a hot topic of social concern, especially in China. The
paper studies hepatitis B virus in maternal blood, breast milk, saliva of hepatitis
B virus infection model (HBV-M) in Hefei city, Anhui province, PRC. HBV-DNA
load and related data in Hefei city are used for risk assessment of the transmission of hepatitis B virus to
provide evidence for evidence-based medicine and scientific guidance of infant
feeding patterns. Methods: On the principle of informed consent,
inpatient hepatitis B maternal blood 695, breast milk, saliva 614,169 copies
were used as the object of analysis, using the ELISA method for the detection
of HBV-M, using real-time fluorescence quantitative PCR detection of HBV-DNA
load. We analyze HBsAg in saliva, milk, the positive rate of HBV-DNA and HBV-M in serum,
saliva, milk, and explore the positive rate of HBV-DNA and serum HBV-DNA load
correlation. Results: At the age of 18 - 44 years old perinatal women,
HBV-DNA positive rates of maternal serum, breast milk, saliva were 157 cases in
A group HBsAg, HBeAg positive: 99.36%, 88.06%, 96.77%; in 312 cases in group B,
HBeAb HBsAg, HBcAb positive: 17.63%, 2.93%, 54.67%; 69 cases in C group HBsAg,
HBcAb positive: 63.77%, 27.27%, 28.57%; D group of 71 patients with simple
HBcAb positive: 12.68%, 3.13%, 0%; E group and 86 cases in control group HBVM:
1.16%, 0%, 0%. According to the serum and milk testing of Group A and Group B, HBV-DNA
chi-square is χ2 = 237.45,
P < 0.01; there is a significant difference in serum and saliva; HBV-DNA chi-square χ2 = 289.49, P < 0.01, the
difference has statistical significance. Conclusion: 1) HBV-DNA load
high maternal blood, breast milk, saliva are potentially persistent hepatitis B
virus infection risk, especially infectious blood. 2) Of
maternal milk, saliva and blood HBV-DNA HBV-DNA load were positively correlated
(r = 0.96; P < 0.01); with the serum HBV-DNA load increasing, breast milk and saliva HBV-DNA positive rates were
increased and infectivity enhanced. 3) Maternal blood, breast milk, saliva
specimens for any HBV-DNA ≥ 1000 copies/ml are not breastfeeding. 4) The mother who carries the hepatitis B virus cannot do maternal infant feeding, and deep kiss intimate
contact, in order to prevent blood, saliva and other ways of infection of
hepatitis B virus. 5) Saliva testing is instead of milk inspection, because saliva
is easier;
Share and Cite:
Huang, C. and Fang, Y. (2019) Maternal Blood Milk Saliva Sample Selection and the Transmission of Hepatitis B Virus Infectious Research.
Open Journal of Obstetrics and Gynecology,
9, 363-370. doi:
10.4236/ojog.2019.93037.