Author(s)

Navjot Kaur Dhillon, Harcharan Singh Dhaliwal

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The present investigation was undertaken to find molecular markers linked to leaf rust resistance genes, Lr9 and Kharchia local mutant KLM4-3B. Preliminary AFLP analysis was carried out with different stocks, a survey of primer combinations with different selective nucleotide indicated that for each primer combination, the number of scorable loci ranged from 34 to 123. Only a limited primer combination used in the set of parental and near isogenic lines showed a high level of polymorphism for AFLP marker. Putative AFLP marker were found to be linked to Lr9, Lr19 and KLM4-3B. The alien genes were readily identified.

AFLP, Leaf Rust, Wheat, Lr9, Isogenic Lines

Dhillon, N. and Dhaliwal, H. (2011) Identification of AFLP Markers Linked to Leaf Rust Resistance Genes Using Near Isogenic Lines of Wheat. American Journal of Plant Sciences, 2, 683-687. doi: 10.4236/ajps.2011.25082.