Author(s)

Allah Jurio Khaskheli¹, Muhammad Ibrahim Khaskheli², Maqsood Ahmad Khaskheli³, Tahmina Shar³, Waqas Ahmad⁴, Umed Ali Lighari³, Muhammad Azeem Khaskheli⁵, Asad Ali Khaskheli⁶, Faisal Hayat Makan⁴

¹Department of Biotechnology, Sindh Agriculture University, Tando Jam, Pakistan.
²Department of Plant Protection, Sindh Agriculture University, Tando Jam, Pakistan.
³State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
⁴Collage of Agriculture and Biotechnology, China Agriculture University, Beijing, China.
⁵Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, China.
⁶Department of Animal Nutrition, Sindh Agriculture University, Tando Jam, Pakistan.

Present study was conducted to assess the regeneration potential and producing mass-clonal seedlings of Rose through shoot tip culture. A total of 40 explants were cultured on each of basal medium supplemented with different concentrations of BAP, NAA and GA. The observations on the survival rate, days taken to initiate the shoots, total number of shoots and length of shoots, initiation of roots, total number of root, length of roots and number of leaves were investigated. Rose regenerated on MS-Basal medium (control) without addition of growth hormones showed significantly (P < 0.05) lower survival rate and did not show shoots up to the end of experiments. MS-Basal medium supplemented with different concentrations of BAP, NAA and GA showed increasing rate of survival. MS-Basal medium supplemented with highest BAP, NAA and GA concentration (MS-SV) has taken least time to initiate the shoots, whilst supplemented in concentrations of 0.5 and/or 1.0 mg/L, respectively revealed more time. Rooted plants were transplanted into the substrate and acclimatized in the laboratory greenhouse (humid cavity). The acclimatization in the humid cavity showed optimistic effect on the number of survived plants.

Rose, Tissue Culture, Explants, BAP, NAA and GA

Khaskheli, A. , Khaskheli, M. , Khaskheli, M. , Shar, T. , Ahmad, W. , Lighari, U. , Khaskheli, M. , Khaskheli, A. and Makan, F. (2018) Proliferation, Multiplication and Improvement of Micro-Propagation System for Mass Clonal Production of Rose through Shoot Tip Culture. American Journal of Plant Sciences, 9, 296-310. doi: 10.4236/ajps.2018.92024.