This study examined the relationship between website quality and online shopping of E-tail stores in Nigeria. A survey research design was adopted. The source of data used in this study was mainly primary data from the issuance of questionnaire and secondary data from the review of relevant literature on both variables which formed the basis for the argument. Out of a total of three hundred and eighty four (384) copies of the questionnaire distributed, three hundred and sixty nine (369) copies were fit for analysis and analyzed using Spearman’s Rank Order Correlation Coefficient with the aid of Statistical Package for Social Sciences (SPSS). The findings of the study reveal amongst others that website aesthetics strongly relates with perceived trust and perceived-ease-of-use of online shoppers in Nigeria. The study concludes that website quality dimensions significantly relates with online shopping of e-tail stores in Nigeria. The study filled the lacuna in the literature of internet marketing as a contribution to knowledge and recommendations amongst others that the e-tail firms should pay keen attention to web security if they must gain more customer patronage as it has the highest positive relationship with online shopping with various suggestions for other researchers interested in the same or similar topic of study.