ABSTRACT
Objective: The aim of study is to research the degradation characteristics of oil degrading Candida tropicalis (G-94). Methods: Effects of temperature, pH, oil concentration, salt concentration, inoculation amount, N and P source on the oil removal rate of G-94 were studied by single factor test. Meanwhile, the effects of temperature, pH, oil concentration and salt concentration on the oil removal rate of G-94 were optimized by orthogonal design. Result: The orthogonal design showed that the oil removal rate of G-94 could reach 25.83%, 28.56% and 30.90% at 10 d, 20 d and 40 d under the optimal conditions which was 25℃, initial pH 8.0, oil concentration 1.0%, salt concentration 0.4%, inoculum amount 4%, the optimal N and P were (NH4)2SO4 and K2HPO4. Conclusion: This experiment studied the degradation characteristics of G-94, which paved the way for the remediation of petroleum contaminated soil.