Author(s)

Yabin Zhan¹, Xingling Tao¹, Shanwen He¹, Shufen Song¹, Jingjing Xing¹, Fangmin Li², Tao Jiang^3*, Lian Ma^1*

¹College of Life Science, Yangtze University, Jingzhou, China.
²College of Chemical and Environmental Engineering, Yangtze University, Jingzhou, China.
³College of Animal Science, Yangtze University, Jingzhou, China.

Objective: The aim of study is to obtain a strain which can effectively degrade oil. Methods: By enrichment, domestication and separation of culture from the sample which comes from Qianjiang Guanghua Oilfield, the pure culture (G-40) was selected from the medium supplemented with oil as the sole source of carbon. The G-40 was preliminarily identified and classified by morphological observation, physiological and biochemical determination and sequence analyses of 16S rDNA. Effects of temperature, pH, oil concentration, salt concentration, inoculation amount, N and P source on the degradation of the oil were studied by single factor test. Meanwhile, the effects of temperature, pH, oil concentration and salt concentration were optimized by orthogonal design. Result: G-40 was isolated and identified as Brevibacillus laterosporus. The orthogonal design showed that the degradation rate of oil could reach 20.81%, 24.34% and 31.15% at 10 d, 20 d and 40 d under the optimal conditions which was 35℃, initial pH 7.3, oil concentration 0.6%, salt concentration 0.5%, inoculum amount 8%, the optimal N and P was (NH₄)₂SO₄ and K₂HPO₄. Conclusion: This study provides microbial resources for bioremediation of petroleum contaminated soil.

Degrading Bacteria, Brevibacillus Laterosporus, Oil Degrading

Zhan, Y. , Tao, X. , He, S. , Song, S. , Xing, J. , Li, F. , Jiang, T. and Ma, L. (2017) Isolation, Identification and Degradation Characteristics of Oil Degrading Bacterial Strain. Open Access Library Journal, 4, 1-12. doi: 10.4236/oalib.1104016.