Author(s)

Franciane S. Marques^1,2, Alysson Luiz M. Da Silva³, Ricardo J. C. Mattoso⁴, Roque Aras⁴, Rogério Jorge B. De Oliveira^1,5, Júlio Cézar De Abreu Santos³, Fabio D. Couto^3,6, Ricardo D. Couto^1,3*

¹PGBSMI/Goncalo Moniz Research Center (CPqGM), FIOCRUZ Foundation Center, Salvador, Brazil.
²Department of Biofunction, Health Institute of Science, Federal University of Bahia/UFBA, Salvador, Brazil.
³Clinical Biochemistry Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Bahia/UFBA, Salvador, Brazil.
⁴Ana Neri Hospital, SESAB and Federal University of Bahia/UFBA, Salvador, Brazil.
⁵Naval Hospital of Salvador, Brazilian Marine Forces, Salvador, Brazil.
⁶Laboratory of Molecular Biology and Genetics at Center of Agricultural, Environmental and Biological Sciences (CCAAB-UFRB), Cruz das Almas, Brazil.

End stage-renal-disease (ESRD) is associated with dyslipidemia and premature atherosclerosis. The study evaluates the effect of hemodialysis (HD) on HDL-remodeling between pre- and post-HD. Was conducted a cross-sectional study with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador, Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3 - 4 hours per week, with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin. HDL Phospholipid-incorporation was measured by ¹⁴C-PL-scintillation-counting, expressed as %¹⁴C-PL/mL/hour. Paraoxonase (PON-1) activity was measured by spectrophotometry using paraoxon as substrate. Cardiovascular risk ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at post-HD on all age groups, but without triglycerides (TG) changes. TG/HDL-C decreased in 30 - 39 and 40 - 49 year (y) at post-HD (p < 0.05). LDL-C/apoB increased in >60 y, after HD (p < 0.05). HDL-C/apo-AI increased in 40 - 49 y (p < 0.05) and >60 (p < 0.01). On the other hand, non-HDL-C/HDL-C reduced in 40 - 49 and >60 y, at post-HD (p < 0.05). The linear-correlation between %¹⁴C-PL-incorporation and non-HDL-C/HDL-C was negative in 30 - 39 y, both at pre-HD (r = -0.90; p = 0.002) and post-HD (r = -0.78; p = 0.022). Linear-correlation between PON-1 and %¹⁴C-PL-incorporation was positive in >60 y, both at pre- (r = 0.63; p = 0.029) and post-HD (r = 0.65; p = 0.022). PON-1 activity increased at pre- (59 ± 30) and post-HD (73 ± 38) in 50 - 59 y (p < 0.05). The %¹⁴C-PL-incorporation was reduced in >60 y (p < 0.05), when compared to pre- and post-HD. ESRD patients undergoing HD shows important changes on lipid-profile, PON-1-activity, cardiac risk ratios and HDL-remodeling. These results demonstrate the influence of HD with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin on lipoprotein metabolism.

Hemodialysis, Dyslipidemia, Cardiovascular Risk Bioindices, PLTP, PON-1, HDL-Remodeling, Low-Flow Polysulfone Dialyzing-Membrane

Marques, F. , Da Silva, A. , Mattoso, R. , Aras, R. , De Oliveira, R. , De Abreu Santos, J. , Couto, F. and Couto, R. (2017) Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and in Vitro Incorporation of Phospholipids. Journal of Biophysical Chemistry, 8, 23-38. doi: 10.4236/jbpc.2017.83003.