ABSTRACT
In order to reveal the phenomenon of R. rugose pollination incompatibility, the full-length cDNA sequence of S Locus
F-box Gene was cloned for the first time from the pollen of Rosa rugose “Zilong wochi” with RT-PCR and RACE methods and
named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino
acids. The derived protein has a molecular weight of 43.7 kD, a calculated pI
of 6.24, an F-box conserved domain at position 343 - 741, and belongs to F-box
family. The derived protein is a Hydrophobicity protein secreted into the cytoplasm.
There is no transmembrane domain and no signal peptide cleavage site, twenty-one
Ser phosphorylation sites, seven Thr phosphorylation sites, seven Tyr
phosphorylation sites, two N-glycosylation sites, and no O-glycosylation sites.
There are 22.25% α-helixes, 31.37% random coil, 32.17% extended
peptide chain, and 14.21% β-corner structure. This protein and the SFB/SLF protein from Rosaceae Prunus fruit, including Prunus speciose, share a sequence
homology of 59% - 61%; all of the proteins contain an F-box conserved domain, two hypervariable regions
HVa, HVb, and two variable regions V1, V2. Furthermore, their phylogenetic
relationships are consistent with their traditional classifications. These
results were meaningful to reveal the molecular mechanism of Rosa rugose pollination
incompatibility and improve the theory and techniques of breeding ornamental Rosa rugose.
Share and Cite:
Wei, Y. , Li, K. , Xing, S. , Zhao, D. , Fu, C. , Zhao, L. , Zang, D. and Yu, X. (2017) Cloning and Bioinformatics Analysis of
Rosa rugose S Locus F-Box Gene (
RrSLF).
American Journal of Plant Sciences,
8, 1550-1559. doi:
10.4236/ajps.2017.87107.