Author(s)

Lulu Han, Junjie Li, Jing Guo, Yan Ma, Xianfeng Guo^*

Forestry College of Shandong Agricultural University, Tai’an, China.

GA20-oxidase (GA20ox) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora. To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA20ox for future gene engineering in peony.

PlGA20ox, Expression Vector, Arabidopsis thaliana, Transformation

Han, L. , Li, J., Guo, J.J., Ma, Y. and Guo, X.F. (2017) Expression Vector Construction and Genetic Transformation of Paeonia lactiflora Gibberellin 20-Oxidase Gene. American Journal of Plant Sciences, 8, 1525-1533. doi: 10.4236/ajps.2017.87105.