Author(s)

Xuejiao Wu, Li Xu, Ganjun Yuan^*, Yimin Wang, Xuejie Xu

College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.

Streptomyces sp. ZZ035 isolated from a folk medicinal soil sample in China showed remarkable antimicrobial activities. During the isolation of secondary metabolites, a white crystal powder (1) was isolated from the broth of this strain. Its nuclear magnetic resonance (NMR) and infrared (IR) spectra indicated that it was a complex composed of triglycerides. Next, six C_15-17 long- chain fatty acids derived from these triglycerides were respectively identified as n-pentadecanoyl, 12-methyltetradecanoyl, 14-methyl pentadecanoyl, palmitoyl, 15-methyl hexadecanoyl and 14-methyl hexadecanoyl using the gas chromatography-mass spectroscopy (GC-MS) technology. Finally, the ¹³C and ¹H assignments of 1 were achieved through the analyses of NMR data. Based on above, their detailed NMR spectroscopic elucidation and meticulous ¹³C, ¹H assignments, especially the split peaks and coupling correlation of protons attached on the glycerol carbons, were performed for distinguishing triglycerides from other glycerides and for the identification of the long-chain fatty acids, and which would be helpful to the qualitative and quantitative analyses of tri-, di-and mono-glycerides.

Nuclear Magnetic Resonance, Triglyceride, Glyceride, Fatty Acid, Bacteria

Wu, X. , Xu, L. , Yuan, G. , Wang, Y. and Xu, X. (2017) Triglycerides Isolated from Streptomyces sp. ZZ035 and Their Nuclear Magnetic Resonance Spectroscopic Characters. Spectral Analysis Review, 5, 1-10. doi: 10.4236/sar.2017.51001.