Author(s)

Sudha Balasubramanian^1*, Charan Thej^1,2*, Ankita Walvekar¹, Priyanka Swamynathan¹, Pawan K. Gupta¹, Raviraja N. Seetharam¹, Anish S. Majumdar¹

¹Stempeutics Research Pvt. Ltd., Bangalore, India.
²Manipal University, Manipal, India.

Background/Purpose: Multipotent bone marrow-derived mesenchymal stromal cells (BMMSC) have been shown to possess the potential for tissue regeneration. The application of mesenchymal stromal cells (MSC)-derived growth factors and cytokines (GF/CKs) has been implicated for the repair and regeneration of the damaged skin that occurs due to aging and exposure to environmental stress factors. Methods: We have used both qualitative and quantitative measurements of the GF/CKs from the conditioned medium (CM) of a pooled population of BMMSC by antibody array analysis as well as by enzyme-linked immunosorbent assay (ELISA). Furthermore, the CM was also used in a variety of in vitro biological assays to measure its protective properties in human skin fibroblasts. Results: We have characterized the secretome of BMMSC by analyzing the composition of the CM using a forty-one growth factor array system. Thirteen of these GF/CK/extra cellular matrix (ECM)/ matrix metalloproteinases (MMP)-inhibitors in the CM were quantified owing to their involvement in skin repair cascade. In addition, we report that the BMMSC-CM was also able to protect dermal fibroblasts against tert-Butyl hydro peroxide (tbOH) induced oxidative stress and ultra violet B (UV-B) radiation induced cell damage. Conclusion: Based on the data presented here, we propose that BMMSC-derived CM may have the potential to promote health and rejuvenation of the aging skin.

Bone Marrow-Derived MSC, Conditioned Medium, Secretome, Oxidative Stress, UV-B Damage

Balasubramanian, S. , Thej, C. , Walvekar, A. , Swamynathan, P. , Gupta, P. , Seetharam, R. and Majumdar, A. (2017) Evaluation of the Secretome Profile and Functional Characteristics of Human Bone Marrow Mesenchymal Stromal Cells-Derived Conditioned Medium Suggest Potential for Skin Rejuvenation. Journal of Cosmetics, Dermatological Sciences and Applications, 7, 99-117. doi: 10.4236/jcdsa.2017.71010.