Expression Vector Construction and Genetic Transformation of Rosa rugosa β-l,3-Glucanase Gene (RrGlu) - American Journal of Plant Sciences

AJPS > Vol.8 No.3, February 2017

Expression Vector Construction and Genetic Transformation of Rosa rugosa β-l,3-Glucanase Gene (RrGlu) ()

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DOI: 10.4236/ajps.2017.83034 1,606 Downloads 2,516 Views Citations

Author(s)

Shutang Xing², Juanjuan Sun¹, Zhihong Peng³, Yanan Fu¹, Lanyong Zhao¹, Zongda Xu¹, Xiaoyan Yu^1*

Affiliation(s)

¹Forestry College of Shandong Agricultural University, Tai’an, China.
²College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China.
³Tai’an Landscape Administration Bureau, Tai’an, China.

ABSTRACT

In order to lay a foundation for researching the function of Rosa rugose (R. rugosa) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121-RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.

KEYWORDS

Rosa rugose, β-l, 3-Glucanase Gene, Expression Vector Construction, Genetic Transformation

Share and Cite:

Xing, S. , Sun, J. , Peng, Z. , Fu, Y. , Zhao, L. , Xu, Z. and Yu, X. (2017) Expression Vector Construction and Genetic Transformation of Rosa rugosa β-l,3-Glucanase Gene (RrGlu). American Journal of Plant Sciences, 8, 495-501. doi: 10.4236/ajps.2017.83034.

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