Author(s)

Yoshiichiroh Mastuba¹, Kazuhiro Okamoto², Yuma Kato³, Nobuo Yamaguchi^1*, Sumiyo Akazawa-Kudoh³

¹Ishikawa Natural Medicinal Products Research Center, Ishikawa, Japan.
²Department of Rehabilitation, Kanazawa Medical University, Uchinada-machi, Japan.
³Department of General Medicine, Kanazawa Medical University, Ishikawa, Japan.

A plant fermentation was carried out by Lactobaccilli against the Rhizome from Pueraria milifica (f-PMF). This material was evidenced by safe in animal toxic study. The main aim of this study was to revise the traditional way of hot water extraction to fermentation so as to use up the original material and finding new activity. We tried to show the new activity through phytoestrogen and immune-competent cells from the host that administrated either of original remedy and the new fermented sample, plus activated water SRE. In mice, compromised host was prepared by cancer chemotherapeutic agent (Mitomycin-C). After administration of f-PMF to immno-suppressed animals, the effects by both samples were augmented by lymphocyte in number and functions, macrophage activities, anti-oxidative activity. However, the intense of effect was much more by fermented one but not by conventional one. The anti-oxidative assay was also carried out ex-vivo system by peritoneal macrophage that we proposed as suitable system for evaluating anti-oxidative assay. In our clinical study by 20 healthy volunteers, granulocyte and lymphocyte ratio was regulated as neutral in peripheral white blood cells, increasing one, two and three weeks after the administration of f-PMF. We have found the significant regulation of blood chemical factors that were important makers for the lifestyle-related diseases. The mechanism of augmentation by probing directory with immuno-electrophoretic method, generating new complement component, especially found by alternative pathway of complement. So we discussed the process concerning designed f-PMF molecule for activation of complement component and bound for the biological activity of each physical component. In a limited condition, fewer numbers of volunteers, the breast size was tending to increase along with the administration time. Including these evidences, we discussed the possibility of this traditional ethnic medicine, originally found and spread in the highland area in Thailand and Myanmar.

Family Pueraria, Fermentation, Lactobacilli, Cancer Chemotherapeutic Agent, Immune-Compromised Host, Lymphocyte, Granulocyte, Ant-Oxidative Activity, Complement, Alternative Complement Activation

Mastuba, Y. , Okamoto, K. , Kato, Y. , Yamaguchi, N. and Akazawa-Kudoh, S. (2017) Fermented Ethnic Medicine (Pueraria mirifica) Designed by Lactobacillus Regulated Leukocyte Subsets via Activation of Complement Components. Open Journal of Rheumatology and Autoimmune Diseases, 7, 65-79. doi: 10.4236/ojra.2017.71006.