Author(s)

Farnaz Sani¹, Giti Borzooeian², Somayeh Kazemnejad³, Sepideh Ebrahimi⁴, Masoomeh Mohamadpour⁵, Sayeh Khanjani³, Mona Latifi¹, Seyed Mojtaba Hosseini², Mahin Salmannejad¹, Fatemeh Aleahmad¹, Hossein Mehraban Jahromi², Mahsa Sani^1,3*

¹Laboratory for Stem Cell Research, Anatomy Department, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
²Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
³Departments of Embryology and Stem Cells, Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
⁴Department of Biochemistry, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.
⁵Anatomy, Histomorphometry and Stereology Research Center, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran.

Menstrual blood stem cells (MensSCs) have enormous potential as a source for cell replacement therapies. Since there is a major concern in utilization of nanofibers in tissue engineering of stem cells, we examined the potential of MensSCs to differentiate into hepatocytes, using different protocols and compare cells, with two-dimensional (2D) and three-dimensional (3D) culture systems. Cell characterization experiments of MensSCs have demonstrated that they are multipotent stem cells similar to mesenchymal stem cells, which can successfully differentiate into osteogenic and adipogenic lineages. The efficiency of the cells on the scaffold was appraised by scanning electron microscopy (SEM), MTT assay, and hematoxylin and eosin (H&E) staining. Thereafter, the differentiation protocols were developed by hepatocyte growth factor (HGF) and oncostatin M (OSM) with serum-supplemented or serum-free culture media up to 30 days. Immunofluorescence analysis and ELISA assay revealed the expression of albumin (ALB) in differentiated cells. Hepatocyte-like cells expressed liver-specific gene such as albumin(ALB), α-fetoprotein (AFP), tyrosine aminotransferase (TAT) and cytochrome P450 subunit 7a1 (Cyp7a1) at mRNA levels. In conclusion, the evidences presented in this study show that the nanofiber scaffold and MensSCs may provide a source of differentiated cells for treatment of liver diseases.

Differentiation, Hepatocyte, Menstrual Blood, Stem Cell, 3D Cultures

Sani, F. , Borzooeian, G. , Kazemnejad, S. , Ebrahimi, S. , Mohamadpour, M. , Khanjani, S. , Latifi, M. , Hosseini, S. , Salmannejad, M. , Aleahmad, F. , Jahromi, H. and Sani, M. (2016) Differentiation of Menstrual Blood Derived Stem Cell (MensSCs) to Hepatocyte-Liked Cell on Three Dimensional Nanofiberscaffold: Poly Caprolacton (PCL). Journal of Biomedical Science and Engineering, 9, 216-225. doi: 10.4236/jbise.2016.94016.