Author(s)

Maria Toloudi, Ioannis Papasotiriou

Research and Development Department, Research Genetic Cancer Centre Ltd., Florina, Greece.

Dendritic cell (DC) maturation approved to be a pivotal process for initiating immunity. Many protocols were established in order the isolated peripheral blood mononuclear cells (PBMCs) from healthy donors to mature into dendritic cells (DCs). The purpose of this study was to present an effective and reliable DC maturation procedure by comparing three different protocols (Interleukin-4/Tumor Necrosis Factor-appha (IL-4/TNFa) DC protocol, Interferon alpha (IFNa) DC protocol and FAST DC protocol). Whole blood was collected from six healthy donors and PBMCs were isolated by Ficoll gradient centrifugation. The counted cells were incubated with the addition of three different cocktails of supplements for appropriate time period. The final mature DC population was examined either by its phenotypic characteristics under light microscope or by measuring the expression of antigen presenting molecules such as CD80 and CD86 by flow cytometry. It was found that the mature DCs, generated from the IL-4/TNFa DC protocol, expressed higher levels of CD80 and CD86. Furthermore, they sharply exhibited their phenotypic hallmarks.

DC Maturation, TNFa, IL-4, IFNa

Toloudi, M. and Papasotiriou, I. (2015) DC Maturation: A Brief Comparison between Three Different Processes. Journal of Cancer Therapy, 6, 871-880. doi: 10.4236/jct.2015.610095.