Determination of Salmonella pullorum with Nanoparticles Immune Based Lateral Flow Strip Assay - Advances in Microbiology

AiM > Vol.5 No.5, May 2015

Determination of Salmonella pullorum with Nanoparticles Immune Based Lateral Flow Strip Assay ()

HTML XML

Download as PDF (Size: 954KB) PP. 364-369

DOI: 10.4236/aim.2015.55038 3,435 Downloads 4,242 Views Citations

Author(s)

Bin Wu^1*, Xiuyun Zhang¹, Wei Pan², Lin Zhang², Fan Zhang²

Affiliation(s)

¹Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, China.
²Chinese Academy of Inspection and Quarantine, Beijing, China.

ABSTRACT

The isolation and culture of conventional detection method of salmonella can not meet the testing requirements of quick and easy detection at the grassroots level. In this study, we prepare the fluorescent nanoparticles as a marker, covalently conjugate with monoclonal antibodies of Salmonella pullorum. The whole Salmonella pullorum antigen and goat anti-mouse antibody sprayed on the nitrocellulose membranes are used as test line and control line. The fluorescence nanoparticles immune based lateral flow strips are made according to the principle of antigen-antibody immune response. The test strips may interpret results within 30 min. The results of the salmonella A, S. agona, S. chester and S. arechavaleta are positive, including, S. agona for weakly positive. After analysis, it is found that in addition to the salmonella of group A, the other positive salmonella are in group B. But it is negative of S. derby, S. rissen, and other 6 kinds of salmonella, with good specificity. The fluorescence nanoparticles immune based lateral flow strips are a little of sample can be detected fast, easily, inexpensive, easy to universal without professional technical personnel detection method. It provides a new detection method for the detections of Salmonella pullorum.

KEYWORDS

Monoclonal Antibody, Nanoparticles, Fluorescence Nanoparticles Immune Based Lateral Flow Strips, Salmonella pullorum

Share and Cite:

Wu, B. , Zhang, X. , Pan, W. , Zhang, L. and Zhang, F. (2015) Determination of Salmonella pullorum with Nanoparticles Immune Based Lateral Flow Strip Assay. Advances in Microbiology, 5, 364-369. doi: 10.4236/aim.2015.55038.

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies