Cloning, Expression Pattern Analysis and Subcellular Localization of Resveratrol Synthase Gene in Peanut (Arachis hypogaea L.) - American Journal of Plant Sciences

AJPS > Vol.5 No.24, November 2014

Cloning, Expression Pattern Analysis and Subcellular Localization of Resveratrol Synthase Gene in Peanut (Arachis hypogaea L.) ()

HTML XML

Download as PDF (Size: 4002KB) PP. 3619-3631

DOI: 10.4236/ajps.2014.524378 3,105 Downloads 4,214 Views Citations

Author(s)

Fanghe Zhu^1*, Jingluan Han^2*, Shumei Liu¹, Xiaoping Chen¹, Rajeev K. Varshney³, Xuanqiang Liang^1*

Affiliation(s)

¹Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
²College of Life Science, South China Agricultural University, Guangzhou, China.
³International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.

ABSTRACT

Resveratrol synthase (RS) is a key enzyme that plays a critical role in the resveratrol synthesis pathway. In this study, six RS genes were isolated and characterized from peanut variety “Zhenzhu Hong” by silico cloning and RT-PCR. Bioinformatics analysis showed that deduced amino acid sequences of the six cloned RS genes were highly conserved with a similarity from 95% to 99% when compared to the RS genes which had been deposited at the GenBank. The results of amino acid sequences analysis showed six RS proteins contained the Chal_Sti_Synt_N and ACP_Syn_III_C domains and can be classified to same family but with different evolutionary distance. Expression pattern analysis by QRT-PCR provided evidence indicating that the mRNA of six RS genes were primarily expressed in the peanut shell at different developmental stages with different expression levels, but only lower levels of them were evident in the peanut kernel. The subcellular localization of RS protein in onion epidermal cell was performed by Agrobacterium tumefaciens-mediated transformation and the green fluorescent was monitored by confocal fluorescence microscopy. The results indicated that, RS1 and RS5 were located in the nucleus and plasma membrane respectively, while the RS2, RS3, RS4 and RS6 were located in both nucleus inner membrane and plasma membrane. The data will provide basic information for elucidating the regulatory mechanisms and enzyme kinetics underlying the RS genes in the resveratrol synthase pathway.

KEYWORDS

Peanut (Arachis hypogaea L.), Resveratrol Synthase Gene, Expression Pattern Analysis, Subcellular Localization, Development

Share and Cite:

Zhu, F. , Han, J. , Liu, S. , Chen, X. , Varshney, R. and Liang, X. (2014) Cloning, Expression Pattern Analysis and Subcellular Localization of Resveratrol Synthase Gene in Peanut (Arachis hypogaea L.). American Journal of Plant Sciences, 5, 3619-3631. doi: 10.4236/ajps.2014.524378.

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies