Author(s)

Mahboubeh Masrournia, Zarrin Es’ haghi, Mostafa Amini

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Hair analysis is used in some branches of alternative medicine as a method of investigation to assist diagnosis. It is very useful when a history of drug use is difficult or impossible to obtain. In this re-search suspended droplet liquid phase microextraction (SDLME) coupled with high-performance liquid chromatography and photodiode array detection (HPLC-DAD) was used for preconcentration and analysis of scopolamine in hair samples. Therefore scopolamine was extracted from 2.0 g hair sample incubated in methanol (5 h, 50°C) and adjusted to pH 7.4 with, Na₂HPO₄–H₃PO₄ buffer solution (donor phase, P₁) into an organic phase (P2) 350 µl n-octanol and then back extracted into a micro drop of aqueous acceptor phase (P3), adjusted at pH 3, with HCL. The extraction time, T₁ (from P₁ to P₂) was 2 min and T₂ (from P₂ to P3) was 30 min. Optimum instrumental conditions were included; A C₁₈ reverse phase column with water-acetonitrile-methanol (80:10:10) as the mobile phase was used and wavelength for UV detec-tion was 205 nm. The linear range was 10 to 10000 ng●mL^–1, enrichment factor, detec-tion limit and relative standard deviation were 77, 0.1 ng●mL^–1 and 5.4 respectively.

Column Liquid Chromatography, Directly Suspended Droplet Liquid Phase Microextraction (SDLME), Scopolamine, Hair Sample

M. Masrournia, Z. haghi and M. Amini, "Liquid Chromatographic Determination of Scopolamine in Hair with Suspended Drop Liquid Phase Microextraction Technique," American Journal of Analytical Chemistry, Vol. 2 No. 2, 2011, pp. 235-242. doi: 10.4236/ajac.2011.22028.