Epidermal Barrier integrity is deteriorated in many skin conditions, including Atopic Dermatitis, and could be responsible for many of the associated clinical symptoms. The aim of this study was to determine the effect of REPAVAR ATOPIC SKIN BODY CREAM EXTREME, product containing “SKIN CALM COMPLEX” on barrier epidermis structure. Morphologic and structural evaluations were conducted on reconstructed human epidermis, by qualitative (hematoxylin/eosin- and immune-staining) and quantitative (MTT assay) techniques. Immature (Age Day 10) and mature (Day 17) sample tissues were treated with the product for 24 hours or 7 days, respectively. Morphological and quantitative analysis revealed an increase in the thickness of the epidermis in both cases after the treatment. The study by immunostaining showed that Product-treated tissues presented excellent localization of: 1) E-cadherin in keratinocytes of the spinous layer; 2) Filaggrin and Loricrin, in the granular layer; and 3) increased expression of Involucrin and Keratin 10 in suprabasal layers and 4) elevated expression of Keratin 5 in the basal layer of the epidermis. The product REPAVAR ATOPIC SKIN BODY CREAM EXTREME increases the epithelial regeneration and differentiation determined by cell proliferation and the expression and localization of specific proteins, improving the epidermal barrier effect.