Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp Litopenaeus vannamei (Crustacea, Decapoda)

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DOI: 10.4236/abb.2014.53034    4,146 Downloads   5,832 Views  Citations

ABSTRACT

The cultivation of the marine species of shrimp Litopenaeus vannamei has emerged as one of the segments that best develop in the Brazilian aquaculture, representing the agribusiness that grew the most in recent years. Regarding the economic importance of the farming of this species in Brazil, further studies on genetic improvement were conducted. A F2 segregating population consisting of 192 samples for each G1 and G2 families, from crossing inbred lines was used for the studies performed in the present work. The genetic linkage analysis was based on polymorphic markers derived from nine AFLP (Amplified Fragment Length Polymorphism) primers. Fourteen genetic linkage groups including 103 segregating polymorphic markers were constructed covering350 cMfor G1 and four genetic linkage groups including 59 markers were constructed covering300 cMfor G2. Simple marker analyses were performed among individuals evaluated phenotypically, finding markers linked to genes that may be potentially important and useful to assess characteristics of economic importance for traits related to weight and the disease IMN (idiopathic muscle necrosis). The statistical model including markers explained major proportion of phenotypic characteristic weight in relation to disease incidence IMN.

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Gonçalves, M. , Regitano, L. , Cruz, C. , Salgado, C. , Freitas, P. , Rocha, J. , Teixeira, A. and Galetti-Junior, P. (2014) Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp Litopenaeus vannamei (Crustacea, Decapoda). Advances in Bioscience and Biotechnology, 5, 273-281. doi: 10.4236/abb.2014.53034.

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