Author(s)

Mambo Moyo

Department of Chemical Technology, Midlands State University, Gweru, Zimbabwe.

Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn²⁺ in aqueous solution. The biosensor designed was able to determine Zn²⁺ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.

Horseradish Peroxidase; Maize Tassel; Multiwalled Carbon Nanotubes; Metal Biosensor; Zn²⁺

Moyo, M. (2014) Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions. Open Journal of Applied Biosensor, 3, 1-7. doi: 10.4236/ojab.2014.31001.