Author(s)

Yu Koyama, Hitoshi Kameyama, Jun Sakata, Takashi Kobayashi, Masahiro Minagawa, Shin-ichi Kosugi, Tadashi Yamamoto, Kohei Akazawa, Toshifumi Wakai

Department of Medical Informatics, Niigata University Graduate School of Medical & Dental Sciences, Niigata, Japan.
Department of Renal Pathology, Institute of Nephrology, Niigata University Graduate School of Medical & Dental Sciences, Niigata, Japan.
Division of Digestive & General Surgery, Niigata University Graduate School of Medical & Dental Sciences, Niigata, Japan.

Backgrounds: Aquaporins (AQPs), the mammalian water channels, have been localized in various organs, including the gastrointestinal (GI) tract. We examined AQPs expression in rat models of massive intestineal resection to determine the functions of AQPs in the GI tract. Methods: Female Sprague-Dawley rats (n = 15) underwent 90% resection of the small intestine, and Female Wistar-Kyoto rats (n = 10), received subtotal colectomy, and were sacrificed following the operations. RNase protection assay and quantitative reverse transcription-polymerase chain reaction (RT-PCR) were performed to measure the AQPs mRNA expression in the GI tract. Immunohistochemistry was performed to confirm AQP8 protein expression. Results: AQP8 mRNA expression (mean ± standard error), was enhanced in the jejunum of the short bowel rats at days 7 and 14 (37.6% ± 1.4% and 18.5% ± 2.4%, respectively, p < 0.01). Enhancement of AQP8 mRNA was also observed in the remnant rectum of the subtotal colectomized rats at both days 21 and 42 (116.1% ± 4.5% and 143.3% ± 7.4%, respectively, p < 0.01). Immunohistochemistry demonstrated enhanced AQP8 expression in the remnant rectum of the subtotal colectomized rats. No intensive change was observed with other AQPs in both models. Conclusions: Our results suggest a compensatory role of AQP8 in the maintenance of intestinal fluid balance.

Aquaporin 8; Short Bowel; Subtotal Colectomy; RNase Protection Assay; QuantitativeRT-PCR

Koyama, Y. , Kameyama, H. , Sakata, J. , Kobayashi, T. , Minagawa, M. , Kosugi, S. , Yamamoto, T. , Akazawa, K. and Wakai, T. (2014) Aquaporin 8 mRNA expression after intestinal resection in rat. Open Journal of Gastroenterology, 4, 62-68. doi: 10.4236/ojgas.2014.42011.