Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells - Advances in Bioscience and Biotechnology

ABB > Vol.4 No.12, December 2013

Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells ()

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DOI: 10.4236/abb.2013.412135 8,832 Downloads 12,865 Views Citations

Author(s)

Si Nga Sou, Karen M. Polizzi, Cleo Kontoravdi

Affiliation(s)

Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, UK.
Department of Life Sciences, Imperial College London, London, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London, UK.
Department of Life Sciences, Imperial College London, London, UK; Centre for Synthetic Biology and Innovation, Imperial College London, London, UK; Centre for Process Systems Engineering, Department of Chemical Engineering, Imperial College London, London, UK.

ABSTRACT

Three transfection reagents, Lipofectamine^® 2000, TransIT-PRO^® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO^® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.

KEYWORDS

Transient Gene Expression; Transfection Efficiency; Chinese Hamster Ovary Cells; mRNA Stability; Temperature Shift

Share and Cite:

Sou, S. , Polizzi, K. and Kontoravdi, C. (2013) Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells. Advances in Bioscience and Biotechnology, 4, 1013-1019. doi: 10.4236/abb.2013.412135.

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