Objective: To explore the mRNA expression of the related
genes of p53, MDM2, vascular endothelial growth factor (VEGF) and hypoxia
inducible transcription factors-1 a (HIF-la) in villous samples of spontaneous
abortion mouse models and normal pregnancy models, and to discuss the effect of
p53, MDM2 on the growth of villous trophoblast cells. Methods: The abortion-prone
CBAXDBA/2 matings were established as the model of spontaneous abortion and the
non-abortion-prone CBAXBALB/c matings as the model of normal pregnancy. Applied
q Real-time PCR method to detect the mRNA expression levels of p53, MDM2, VEGF
and HIF-la in villous samples of spontaneous abortion mouse models and normal
pregnancy models. Results: The relationship of the mRNA expression level of p53,
MDM2, VEGF and HIF-la in vinous samples of spontaneous abortion mouse models:
in the villous samples of spontaneous abortion mouse models, the expression of
p53 was positively correlated with the expression of MDM2, HIF-la (r = 0.35; r
= 0.63), and the relationship was significant (P = 0.01; P < 0.001); but
negatively correlated to the expression of VEGF (r = ?0.30), and the
relationship was significant (P = 0.03). The expression of MDM2 was positively
correlated with the expression of HIF-la (r = 0.28), and the relationship was
significant (P = 0.04); and negatively correlated with the expression of VEGF
(r = ?0.08), but the relationship was not significant (P = 0.57). The
expression of HIF-la was negatively correlated with the expression of VEGF (r =
?0.37), and the relationship was significant (P = 0.007). The relationship of
the mRNA expression level of p53,MDM2, VEGF and HIF-1 a in vinous samples of
normal pregnancy models: in the vinous samples of normal pregnancy models, the
expression of p53 was positively correlated with the expression of MDM2, VEGF
and HIF-la (r = 0. 31; r = 0. 48; r = 0. 67), and the relationship was
significant (P = 0.03; P = 0.003; P < 0.001). The expression of MDM2 was
positively correlated with the expression of VEGF (r = 0. 23), but the
relationship was not significant (P = 0.11); and negatively correlated with the
expression of HIF-la (r = ?0.03), but the relationship was not significant (P =
0.84). The expression of HIF-la was positively correlated with the expression
of VEGF (r = 0. 35), and the relationship was significant (P = 0.01). Conclusion:
angiogenesis reduces in villous samples of spontaneous abortion mouse model,
P53 and MDM2 involve in angiogenesis in villous samples, unlikely p53, and MDM2
have effects on normal early pregnancy villous angiogenesis and when the cell
DNA damages or hypoxia exacerbates, it can induce high expression of p53, MDM2,
inhibit angiogenesis in villous samples in early pregnancy. P53, MDM2
generegulate villous trophoblast cell growth by adjusting expression of HIF-1a
and VEGF gene, finally influences pregnancy.