This study proposes an innovative design method for functional optical film microstructures used in displays and applies this design to LCD backlighting to replace multi-layer optical film functional integration with composite optical film. We design a novel optical film microstructure based on light uniformity and wide-angle lumination distribution and determine the optimal optical microstructure parameters by combining the global optimization of a genetic algorithm with ray tracing. The purpose of this study is to develop substitutes for traditional multi-layer prism brightness enhancers and light-diffusing film stacks and to examine the structural changes during calculations and summarize the characteristics. In this study, we focus on determining the optimal light uniformity of new optical film microstructures. The seven-inch LED backlight module of the method proposed in this study achieved 94.59% uniformity and 168° lumination distribution while reducing thickness by 66% to 82% compared to a traditional multi-layer optical film stack.