Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepacia NRRL B-2320 - Advances in Microbiology

AiM > Vol.3 No.6, October 2013

Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepacia NRRL B-2320 ()

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DOI: 10.4236/aim.2013.36064 4,103 Downloads 6,577 Views Citations

Author(s)

Kasturi Dutta, Veeranki Venkata Dasu, Krishnamoorthy Hegde

Affiliation(s)

.
Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, India.

ABSTRACT

The development of medium for the production of cutinase from Pseudomonas cepacia NRRL B 2320 was carried out using Plackett-Burman experimental design followed by central composite design. The medium components were screened by Plackett-Burman experimental design which suggested that cutin, peptone, KCl and MgSO₄·7H₂O have influenced the cutinase production significantly with very high confidence levels. The concentration levels of these four components were optimized using 2⁴ full factorial central composite design. An optimum combination of 10.06 g·L^-1of cutin, 17.77 g·L^-1 of peptone, 0.635 g·L^-1 of KCl and 5.455 g·L^-1 of MgSO₄·7H₂O in the medium gave a maximum cutinase activity of 336 U·mL^-1. An overall 2 fold increase in the production of cutinase was observed in the optimized medium. Growth and production of cutinase from P. cepacia NRRL B 2320 have been studied in shake flask and batch bioreactor. Time course of cell growth and enzyme production was fitted to the existing kinetic models reported in the literature to estimate the biokinetic parameters. These models suggested that the production of cutinase is growth associated in shake flask and it is a mixed growth type in a batch bioreactor.

KEYWORDS

Cutinase; Kinetics; Bioprocessing; Optimization; Parameter Identification

Share and Cite:

K. Dutta, V. Dasu and K. Hegde, "Development of Medium and Kinetic Modeling for Enhanced Production of Cutinase from Pseudomonas cepacia NRRL B-2320," Advances in Microbiology, Vol. 3 No. 6, 2013, pp. 479-489. doi: 10.4236/aim.2013.36064.

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