Increased turnover of organic matter as a result of soil disturbance (e.g. by soil tillage) is described in principle, but the direct influence of soil disturbance on soil P turnover especially for organic farming systems has not been sufficiently proven. The objective of the study was to evaluate the short term effect of soil disturbance on different soil P fractions in a soil shaking experiment. Four soils were incubated for 10 days in the dark with three different disturbance treatments: 1) no disturbance, 2) overhead shaking for 2 h at the beginning of the experiment and 3) continuous overhead shaking at 5 r. p. m. The four investigated soils were: 1) a silty loam soil with long term bio-compost application and 2) the corresponding soil without bio-compost application, 3) a long-term organically managed clay loam soil and 4) a clay loam soil with long time application of pig manure, all not and from Baden-Württemberg, Germany. We determined NaHCO₃-, NaOH- and H₂SO₄-extractable inorganic and organic P fractions (P_i and P_o, resp.) in a sequential extraction. Furthermore, the potentially plant available P as Calcium-acetate-lactate-extractable P (CAL-P) and P extractable by electro-ultra-filtration (EUF-P), and aqua regia extractable total P (P_T) were determined. Furthermore, we determined microbial biomass carbon (MBC), nitrogen (MBN) and phosphorus (MBP), and acid phosphatase activity in soil. The organically managed soil had the highest P_T contents (1300 mg·kg^-1). The soil with pig manure application had the smallest potentially labile P fractions (NaHCO₃-P_i and -P_o and NaOH-P_i). The ecologically managed soil had the biggest organic P fractions (114 mg·kg^-1 NaHCO₃-P_o and 463 mg·kg^-1 NaOH-P_o), but, this soil was the lowest in CAL-P (5 mg·kg^-1). Short term soil disturbance had effects on labile organic P fractions of two of the four analyzed soils, but inorganic P was rather unaffected. In the compost amended COMP(+) soil, there was an incorporation of P from the less available NaOH-P fractions into the more available NaHCO₃-P_o fraction. However, if taking all investigated soils and treatments into account, the effects of soil disturbance were limited and not consistent.