Molecular modeling as a tool in studying peptide-substrate interactions provides insight on peptide adsorption conformation, adsorption energy, and stability of the peptide-inorganic interface. This work investigates the hydration and interaction of cell-adhesion peptides, specifically RGD and YIGSR, with the hydroxyapatite surface and TiO₂ surface in cluster and periodic boundary condition approaches. The comparison of adsorption energies of RGD and YIGSR on both Hydroxyapatite (HA) and TiO₂ surfaces reveals the similarities in adsorption energy and orientation pattern of peptides on both surfaces. The models demonstrate that initial peptide orientation affects adsorption energy for both. YIGSR is consistently more strongly adsorbed to HA-(001) surfaces and steps than RGD for both the surfaces. In addition, RGD maintained its “hairpin”-like structure during adsorption on a flat HA-(001) surface, and a slightly “relaxed hairpin” structure on TiO₂ (110) surface. Adsorption energies of RGD on TiO₂ (110) surface are significantly more favorable compared to HA-(001) surface, suggesting potential role of TiO₂ as biomedical implants when tissue regeneration occurs via cell signaling.