A robust protein extraction method for two dimensional electrophoresis of silkworm proteins - Advances in Bioscience and Biotechnology

ABB > Vol.4 No.4, April 2013

A robust protein extraction method for two dimensional electrophoresis of silkworm proteins ()

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Download as PDF (Size: 1368KB) PP. 584-589

DOI: 10.4236/abb.2013.44076 4,481 Downloads 7,085 Views Citations

Author(s)

Lvgao Qin, Haifeng Shi, Hengchuan Xia, Yong Wang, Liang Chen, Qin Yao, Keping Chen

Affiliation(s)

Institute of Life Sciences, Jiangsu University, Zhenjiang, China.
School of Life Science, Sichuan University, Chengdu, China.

ABSTRACT

Silkworm (Bombyx mori) is a species of agricultural importance, as well as a model organism for Lepidoptera insects. Proteomic method has been widely used in silkworm research, and a robust mass spectrometry-compatible protein extraction method is urgently needed. In this study, we adapted phenol extraction method to extract silkworm midgut protein, and coupled this method with pH 5 - 8 gel strip for two dimensional electrophoresis. The phenol extraction method significantly increased the resolution, as well as greatly reduced the background of two dimensional electrophoresis gels. In addition, this method was well compatible with mass spectrometry analysis. This is the first report that phenol extraction method is used for silkworm midgut protein extraction, and may be applied in other researches.

KEYWORDS

Keywords: Silkworm; Proteomic; Mass Spectrometry; Protein Extraction; Two Dimensional Electrophoresis

Share and Cite:

Qin, L. , Shi, H. , Xia, H. , Wang, Y. , Chen, L. , Yao, Q. and Chen, K. (2013) A robust protein extraction method for two dimensional electrophoresis of silkworm proteins. Advances in Bioscience and Biotechnology, 4, 584-589. doi: 10.4236/abb.2013.44076.

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