Author(s)

Renfang Chen, Zehua Zhang, Weiting Sun, Yun Fu, Quncai Zhang, Liang Wei, Yanmei Liang, Ruocheng Dong

College of Biotechnology, Southwest University/Key Sericultural Laboratory of Ministry of Agriculture, Chongqing, China.

This article mainly studied Humulus scandens’ ITS sequence, its system position and its divergent time. ITS is 624 bp with a GC percentage content of 57.21%. It only has the variation in 585 (C changes T), systematic position in Humulus lupulus (GenBank: EF136401) and Humulus scandens (GenBank: FJ980285), Humulus lupulus (GenBank: DQ005990), with Humulus scandens (GenBank: FJ980285), Humulus lupulus (GenBank: DQ005990) has the near sibship. Humulus in Moraceae family system position between Cudrania tricuspidata and Artocarpeae, support the Cannabiodeae promotion for Cannabinaceae. The divergent time of Humulus is 70.88 mya in Moraceae, Humulus scandens and Humulus lupulus (GenBank: EF136401) is 12.78 mya, with Humulus scandens (GenBank: FJ980285), Humulus lupulus (GenBank: DQ005990) is 1.10 mya. In the Moraceae branch’s molecular systematics research, the suitable choice is to choose Humulus scandens as Moraceae’s outgroup.

Moraceae; Humulus scandens; ITS; Systematic Position; Divergent Time

R. Chen, Z. Zhang, W. Sun, Y. Fu, Q. Zhang, L. Wei, Y. Liang and R. Dong, "System Position and Divergent Time of Based on ITS Sequence Humulus scandens," American Journal of Plant Sciences, Vol. 3 No. 10, 2012, pp. 1470-1475. doi: 10.4236/ajps.2012.310177.