Author(s)

Chen Qian, Yanjuan Yuan, Xuejun He, Jing Liu, Qing Shao, Hua Wu, Hongqun Qiao

Jiangsu center for safety evaluation of drugs, Nanjing, China.
School of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
School of Pharmaceutical Sciences, Nanjing University of Technology, Nanjing, China.

In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra- and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.

Dencichine; (L)-2-Amino-3-(Carboxymethylthio)Propionic Acid; Hplc-Ms/Ms; Pharmacokinetic;Ammonium Acetate Solution-Acetonitrile (35:65, V/V) Mobile Phase

C. Qian, Y. Yuan, X. He, J. Liu, Q. Shao, H. Wu and H. Qiao, "A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics," American Journal of Analytical Chemistry, Vol. 3 No. 8, 2012, pp. 596-603. doi: 10.4236/ajac.2012.38078.