Author(s)

Alejandro Yam-Puc, Elidé Avilés-Berzunza, Manuel J. Chan-Bacab, Luis Manuel Peña-Rodríguez, Gregorio Godoy-Hernández

Centro de Investigación Científica de Yucatán, A.C. Laboratorio de Química Orgánica, Unidad de Biotecnología, Mérida, México.
Departamento de Microbiología Ambiental y Biotecnología, Universidad Autónoma de Campeche, Campeche, México.
Unidad de Bioquímica y Biología Molecular, Mérida, México.

Sections of hypocotyls, roots and leaves from Pentalinon andrieuxii plantlets were transiently transformed with Agrobacterium tumefaciens LBA4404 bearing the binary plasmid pCAMBIA2301 with an interrupted β-Glucuronidase (GUS) gene. Histochemical GUS assays showed transient gene expression in all infected tissues, being older roots those which displayed the most intense GUS staining. To our knowledge, this is the first report of Pentalinon andrieuxii susceptibility to Agrobacterium tumefaciens-mediated genetic transformation.

β-Glucuronidase; Agrobacterium tumefaciens; Kanamycin; Apocynaceae

Yam-Puc, A. , Avilés-Berzunza, E. , Chan-Bacab, M. , Peña-Rodríguez, L. and Godoy-Hernández, G. (2012) Agrobacterium-mediated transient transformation of Pentalinon andrieuxii Müll. Arg.. Advances in Bioscience and Biotechnology, 3, 256-258. doi: 10.4236/abb.2012.33035.