Author(s)

Abdul Wahab Khan, Umme Salma Zohora, Mohammad Shahedur Rahman, Masahiro Okanami, Takashi Ano

Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan.
Department of Biotechnological Science, Faculty of Biology-Oriented Science and Technology, Kinki University, Kinokawa, Japan.
Department of Biotechnology and Genetic Engineering, Faculty of Biological Sciences, Jahangirnagar University, Dhaka, Bangladesh.
Proximo Biotech Ltd., Dinajpur, Bangladesh.Chemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan.

The present study was conducted with an aim to scale up the production of iturin A using soybean curd residue (okara). Iturin A was produced by indigenous bacterial strain Bacillus subtilis RB14-CS through glass column reactor (GCR) under solid state fermentation (SSF) was characterized. The enhanced iturin A production was observed with respect to enhanced substrate bed height when SSF was conducted in Erlenmeyer flask. To check the effect of substrate bed height on iturin A production under SSF of okara, GCR was introduced. Substrate bed height of 15 cm was suitable for iturin A production which was about 2700 mg/kg wet substrate. The observed iturin A production by the aerobic bacteria Bacillus subtilis in nearly anaerobic condition in such high substrate bed for SSF is a wonderful finding for development of SSF system in future.

Solid State Fermentation; Okara; Iturin A; Glass Column Reactor

Khan, A. , Zohora, U. , Rahman, M. , Okanami, M. and Ano, T. (2012) Production of iturin A through glass column reactor (GCR) from soybean curd residue (okara) by Bacillus subtilis RB14-CS under solid state fermentation (SSF). Advances in Bioscience and Biotechnology, 3, 143-148. doi: 10.4236/abb.2012.32021.