Effects of Agrobacterial rol-Genes on the Thermodynamic and Structural Features of Starches Extracted from Potato Microtubers

Luybov A. Wasserman, Nina P. Aksenova, Tatiyana N. Konstantinova, Lidiya I. Sergeeva, Svetlana A. Golyanovskaya, Alexey V. Krivandin, Georgy A. Romanov

Emmanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
imiryazev Institute of Plant Physi- ology, Russian Academy of Sciences, Moscow, Russia.
Timiryazev Institute of Plant Physi- ology, Russian Academy of Sciences, Moscow, Russia.
Timiryazev Institute of Plant Physi- ology, Russian Academy of Sciences, Moscow, Russia;Laboratory of Plant Physiology, Wageningen University, Wageningen, The Netherlands..

DOI: 10.4236/fns.2014.53031                

Abstract

Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a source of single-node stem cuttings. The structure of native starch in tubers formed on cuttings was determined using methods of X-ray scattering and differential scanning microcalorimetry (DSC). It was found that in starch from tubers of rolB plants the melting temperature of crystalline lamella was lower and their thickness was less than that in wild-type potato. In tubers of rolC plants starch differed from starch in wild-type plants by a higher melting temperature, reduced melting enthalpy, and a greater thickness of crystalline lamellae. The melting of starch from tubers of rolC plants proceeded as the melting of two independent crystalline structures with melting temperatures of 338.0°K and 342.8°K. Overall data show that starches of different structure can be obtained by using transgenic approach.

Keywords

Solanum tuberosum; Transgenic Potato; Starch; rol Genes; Tubers; Crystalline Lamellae; Melting Temperature

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	S. D. Jackson, “Multiple Signaling Pathways Control Tuber Induction in Potato,” Plant Physiology, Vol. 119, No. 1, 1999, pp. 1-8. http://dx.doi.org/10.1104/pp.119.1.1
[2]	D. J. Hannapel, “Signalling the Induction of Tuber Formation,” In: D. Vreugdenhil, Ed., Potato Biology and Biotechnology: Advances and Perspectives, Elsevier, Amsterdam, 2007, pp. 237-256. http://dx.doi.org/10.1016/B978-044451018-1/50054-3
[3]	E. E. Ewing, “The Role of Hormones in Potato (Solanum tuberosum L.) Tuberization,” In: P. J. Davies, Ed., Plant Hormones. Physiology, Biochemistry and Molecular Biology, Kluwer, Dordrecht, 1995, pp. 698-724.
[4]	M. Rodríguez-Falcón, J. Bou and S. Prat, “Seasonal Control of Tuberization in Potato: Conserved Elements with the Flowering Response,” Annual Review of Plant Biology, Vol. 57, 2006, pp. 151-180. http://dx.doi.org/10.1146/annurev.arplant.57.032905.105224
[5]	N. P. Aksenova, T. N. Konstantinova, S. A. Golyanovskaya, L. I. Sergeeva and G. A. Romanov, “Hormonal Regulation of Tuber Formation in Potato Plants,” Russian Journal of Plant Physiology, Vol. 59, No. 4, 2012, pp. 451-466. http://dx.doi.org/10.1134/S1021443712040024
[6]	G. Ooms, D. Twel, M. E. Bossen, J. H. C. Hoge and M. Burrell, “Developmental Regulation of Ri T1-DNA Gene Expression in Roots, Shoots and Tubers of Transformed Potato (Solanum tuberosum cv Diseree),” Plant Molecular Biology, Vol. 6, No. 5, 1986, pp. 321-330. http://dx.doi.org/10.1007/BF00034939
[7]	T. Schmülling, M. Fladung, K. Grossmann and J. Schell, “Hormonal Content and Sensitivity of Transgenic Tobacco and Potato Plants Expressing rol-Genes of Agrobacterium rhizogenes T-DNA,” The Plant Journal, Vol. 3, No. 3, 1993, pp. 371-382. http://dx.doi.org/10.1046/j.1365-313X.1993.t01-20-00999.x
[8]	M. Fladung, A. Ballvora and T. Schmülling, “Constitutive of Light Regulated Expression of the rolC Gene in Transgenic Potato Plants has Different Effects on Yield Attributes and Tuber Carbohydrate Composition,” Plant Molecular Biology, Vol. 23, No. 4, 1993, pp. 749-757. http://dx.doi.org/10.1007/BF00021530
[9]	E. V. Grishunina, “Effects of rol-Genes on Carbohydrate Metabolism during Tuberization in Potato Plants,” Ph.D. Thesis, Inst. Plant Physiol. RAS, Moscow, 2006. (in Russian)
[10]	I. A. Gukasyan, S. A. Golyanovskaya, E. V. Grishunina, T. N. Konstantinova, N. P. Aksenova and G. A. Romanov, “Effect of rol-Transgenes, IAA, and Kinetin on Starch Content and the Size of Starch Granules in Tubers of in Vitro Potato Plants,” Russian Journal of Plant Physiology, Vol. 52, No. 6, 2005, pp. 809-813. http://dx.doi.org/10.1007/s11183-005-0119-1
[11]	J. P. Davis, N. Supatcharee, R. L. Khandelwal and R. N. Chibbar, “Synthesis of Novel Starches in Planta: Opportunities and Challenges,” Starch-Starke, Vol. 55, No. 3-4, 2003, pp. 107-120. http://dx.doi.org/10.1002/star.200390036
[12]	L. I. Sergeeva, S. M. De Bruijon, E. A. M. Koot-Grosveld, O. Navratil and D. Vreugdenhil, “Tuber Morphology and Starch Accumulation are Independent Phenomena: Evidence from ipt-Transgenic Potato Lines,” Physiologia Plantarum, Vol. 108, No. 4, 2000, pp. 435-443. http://dx.doi.org/10.1034/j.1399-3054.2000.100414.x
[13]	M. Richter, S. Augustat and F. Schierbaum, “Ausgewahlte Methoden der Starkechemie,” Wissenschaftlliche Verlagsgesellschaft, GmbH, Stuttgart, 1968.
[14]	Y. I. Matveev, J. J. G. van Soest, C. Nieman, L. A. Wasserman, V. A. Protserov, M. G. Ezernitskaja and V. P. Yuryev, “The Relationship between Thermodynamic and Structural Properties of Low and High Amylose Maize Starches,” Carbohydrate Polymers, Vol. 44, No. 2, 2001, pp. 151-160. http://dx.doi.org/10.1016/S0144-8617(00)00211-3
[15]	V. P. Yuryev, L. A. Wasserman, N. R. Andreev and V. B. Tolstoguzov, “Structural and Thermodynamic Features of Lowand High-Amylose Starches. A Review,” In: V. P. Yuryev, A. Cesaro and W. Bergthaller, Eds., Starch and starch Containing Origins—Structure, Properties and New Technologies, Nova Science Publishers, New York, 2002, pp. 23-56.
[16]	C. G. Billarderis, C. M. Page, L. Slade and R. R. Sirett, “Thermal Behaviour of Amylose-Lipid Complexes,” Carbohydrate Polymers, Vol. 5, No. 5, 1985, pp. 367-371. http://dx.doi.org/10.1016/0144-8617(85)90044-X
[17]	P. L. Privalov and S. A. Potekhin, “Scanning Microcalorimetry in Studying Temperature-Induced Changes in Proteins,” Methods in Enzymology, Vol. 131, 1986, pp. 451.
[18]	V. A. Bershtein and V. M. Egorov, “Differential Scanning Calorimetry of Polymers: Physics, Chemistry, Analysis, Technology,” Ellis Horwood, New York, London, Toronto, Sydney, Singapore, 1994.
[19]	I. I. Bocharnikova, L. A. Wasserman, A. V. Krivandin, J. Fornal, W. Blaszczak, V. Ya. Chernykh, A. Schiraldi and V. P. Yuryev, “Structure and Thermodynamic Melting Parameters of Wheat Starches with Different Amylose Content,” Journal of Thermal Analysis and Calorimetry, Vol. 74, No. 3, 2003, pp. 681-695. http://dx.doi.org/10.1023/B:JTAN.0000011001.02981.88
[20]	M. A. Whittam, T. R. Noel and S. Ring, “Melting and Glass/Rubber Transition of Starch Polysaccharides,” In: E. Dickinson, Ed., Food Polymers, Gels and Colloids, Royal Society of Chemistry, London, 1991, pp. 277-278. http://dx.doi.org/10.1533/9781845698331.277
[21]	V. P. Yuryev, A. V. Krivandin, V. I. Kiseleva, L. A. Wasserman, N. K. Genkina, J. Fornal, W. Blaszczak and A. Schiraldi, “Structural Parameters of Amylopectin Clusters and Semi-Crystalline Growth Rings in Wheat Starches with Different Amylose Content,” Carbohydrate Research, Vol. 339, No. 16, 2004, pp. 2683-2691. http://dx.doi.org/10.1016/j.carres.2004.09.005
[22]	N. P. Aksenova, T. N. Konstantinova, S. A. Golyanovskaya, T. Schmülling, J. Kossmann, L. Willmitzer and G. A. Romanov, “In Vitro Growth and Tuber Formation by Transgenic Potato Plants Harboring rolC or rolB Genes under Control of the Patatin Promoter,” Russian Journal of Plant Physiology, Vol. 46, No. 4, 1999, pp. 513-519.
[23]	N. P. Aksenova, T. N. Konstantinova, S. A. Golyanovskaya, T. Schmülling, J. Kossmann, L. Willmitzer and G. A. Romanov, “Transformed Potato Plants as a Model for Studying the Hormonal and Carbohydrate Regulation of Tuberization,” Russian Journal of Plant Physiology, Vol. 47, No. 3, 2000, pp. 370-379.
[24]	V. A. Protserov, L. A. Wasserman, R. F. Tester, S. J. J. Debon, M. G. Ezernitskaja and V. P. Yuryev, “Thermodynamic and Structural Properties of Starches Extracted from Potatoes Grown at Differential Environmental Temperatures,” Carbohydrate Polymers, Vol. 49, No. 3, 2002, pp. 271-279. http://dx.doi.org/10.1016/S0144-8617(01)00332-0
[25]	Ch. Gernat, S. Radosta, H. Anger and G. Damaschun, “Crystalline Parts of Three Different Conformations Detected in Native and Enzymatically Degraded Starches,” Starch/Starke, Vol. 45, No. 9, 1993, pp. 309-314. http://dx.doi.org/10.1002/star.19930450905
[26]	T. Ya. Bogracheva, V. J. Morris, S. G. Ring and C. L. Hedley, “The Granular Structure of C-Type Starch and Its Role in Gelatinization,” Biopolymers, Vol. 45, No. 4, 1998, pp. 323-332. http://dx.doi.org/10.1002/(SICI)1097-0282(19980405)45:4<323::AID-BIP6>3.0.CO;2-N
[27]	P. Cairs, T. Bogracheva, S. G. Ring, L. L. Hedley and V. J. Morris, “Determination of the Polymorphic Composition of Smooth Pea Starch,” Carbohydrate Polymers, Vol. 31, No. 3-4, 1997, pp. 275-282. http://dx.doi.org/10.1016/S0144-8617(96)00115-4
[28]	G. O. Kozhevnikov, V. A. Protserov, N. E. Pavlovskaya, L. V. Golischkin, V. N. Milyaev and V. P. Yuryev, “Changes of Thermodynamic and Structural Properties of Wrinkled Pea Starches (Z-301 and Paramazent varieties) During Biosynthesis,” Starch/Starke, Vol. 53, No. 5, 2001, pp. 201-210. http://dx.doi.org/10.1002/1521-379X(200105)53:5<201::AID-STAR201>3.0.CO;2-Q
[29]	P. J. Jenkins, R. E. Cameron and A. M. Donald, “A Universal Feature in the Structure of Starch Granules from Different Botanical Sources,” Starch/Starke, Vol. 45, No. 12, 1995, pp. 417-420. http://dx.doi.org/10.1002/star.19930451202
[30]	L. J. Sweetlove, B. Müller-Rober, L. Willmitzer and S. A. Hill, “The Contribution of Adenosine 5’-Diphosphoglucose Pyrophosphorylase to the Control of Starch Synthesis in Potato Tubers,” Planta, Vol. 209, No. 3, 1999, pp. 330-337. http://dx.doi.org/10.1007/s004250050640