Screening and Fermentation Characteristics of PHA Bacteria in Activated Sludge ()
Abstract
Using sludge at the bottom of Hengshui Lake as samples,
and screened out the SJ-9 strain with PHA synthesis capability
by enrichment, purification, Nile blue staining, far-infrared spectroscopy and
gas qualitative and quantitative analysis. The infrared scanning of SJ-9
bacteria product showed that this product was middle chain PHA. Optimization of
SJ-9 fermentation conditions obtained the optimal conditions with optimal
carbon source of sodium butyrate, optimal C/N of 35, optimal fermentation time
of 120 h, and optimal initial pH for culture substrate of 7.0. 10 L fermenter
amplification culture under these conditions showed consistent results
with flask culture, and the bacteria and PHA had the highest yields at 120 h,
with the maximal yields of 6.81 and 2.36 g/L, respectively.
Share and Cite:
Yang, F. and Zhu, R. (2013) Screening and Fermentation Characteristics of PHA Bacteria in Activated Sludge.
Engineering,
5, 467-471. doi:
10.4236/eng.2013.510B096.
Conflicts of Interest
The authors declare no conflicts of interest.
References
|
[1]
|
C. Suchada, “Current Trends in Biodegradable Polyhydroxyalkanoates,” Journal of Bioscience and Bioengineering, Vol. 110, 2010, pp. 621-632.
http://dx.doi.org/10.1016/j.jbiosc.2010.07.014
|
|
[2]
|
B. Hazer and C. Steinb, “Increased Diversification of Polyhydroxyalkanoates by modiFication Reactions for Industrial and Medical Applications,” Applied Microbiology and Biotechnology, Vol. 74, 2005, pp. 1-12.
http://dx.doi.org/10.1007/s00253-006-0732-8
|
|
[3]
|
R. W. Lenz and H. Merchessaultr, “Bacterial Polyesters: Biosynthesis Biodegradable Plastics and Biotechnology,” Biomacromolecules, Vol. 6, 2005, p. 1.
http://dx.doi.org/10.1021/bm049700c
|
|
[4]
|
J. M. Cavalheiro and M. C. Almeida, “Poly (3-Hydroxybutyrate) Pro-duction by Cupriavidus Necator Using Waste Glycerol,” Process Biochemistry, Vol. 44, 2009, pp. 509-515.
http://dx.doi.org/10.1016/j.procbio.2009.01.008
|
|
[5]
|
J. H. Song, C. O. Jeon and H. Choim, “Polyhydroxyalkanoate (PHA) Production Using Waste Vegetable Oil by Pseudomonas sp. Strain Dr2,” Journal of Microbiology and Biotechnology, Vol. 18, 2008, pp. 1408 -1415.
|
|
[6]
|
A. J. Verlinden and D. J. Hill, “Bacterial Synthesis of Biodegradable Polyhydroxyalkanoates,” Journal of Applied Microbiology, Vol. 102, 2007, pp. 1227-1229.
http://dx.doi.org/10.1111/j.1365-2672.2007.03335.x
|