α-Galactosyl Phytosphingosine 2,6’-Diamide as an Inducer of Invariant Natural Killer T Cell

Ying-Cheng Huang¹, Wei-Ting Chen², Shu-Fan Tien², Ho-Lien Huang², Chun-Nan Yeh³, Kun-I Lin^4*, Chung-Shan Yu⁵

¹Department of Neurosurgery, Chang Gung Memorial Hospital at Linkou, Chang Gung University, Taoyuan City.
²Department of Biomedical Engineering and Environmental Sciences, National Tsing-Hua University, Hsinchu City.
³Department of Surgery, Chang Gung Memorial Hospital at Linkou, Chang Gung University, Taoyuan City.
⁴Department of Obsterics and Gynecology, Chang Bing Show Chwan Memorial Hospital, Changhua City.
⁵Institute of Nuclear Engineering and Science, National Tsing-Hua University, Hsinchu City.

DOI: 10.4236/ojmc.2013.33008                

Abstract

Four a-galactosyl phytosphingosine 2,6’-diamide analogs were prepared from 2,6’-diamino a-galactosylphytosphingosine and the aromatic-bearing carboxylic acids. After purification with High Performance Liquid Chromatography, a flowcytometry for the four compounds for stimulation of human Va24+/Vb11+ NKT cell populations was carried out. Additional keto groups on the acyl chains of the 2,6’-diamide compound was associated with the enhanced stimulating effect.

Keywords

Phytosphingosine; HPLC; Keto; Flow Cytometry; Simulation

Conflicts of Interest

The authors declare no conflicts of interest.

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