Residual Effects of Sucrose and Hormonal Treatments of the Tuberization Medium on in Vitro Germination of Potato (Solanum tuberosum L.) Microtubers

Abraham Diémé, Mame Abdou Nahr Sambe, Emile Codjo Agbangba, Mame Ourèye Sy

Laboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences & Techniques, Université Cheikh Anta Diop, Dakar, Sénégal.
Laboratoire Campus de Biotechnologies Végétales, Département de Biologie Végétale, Faculté des Sciences & Techniques, Université Cheikh Anta Diop, Dakar, Sénégal;.
Laboratoire d’Ecologie Végétale et d’Ecohydrologie, Département de Biologie Végétale, Faculté des Sciences & Techniques, Université Cheikh Anta Diop, Dakar, Sénégal..

DOI: 10.4236/ajps.2013.49230                

Abstract

The residual effects of sucrose concentrations (80 or 100 g·L^-1) and hormonal treatments (BAP + Kinetin or Coumarin) of tuberization medium on in vitro microtubers germination of three potato varieties (Solanum tuberosum L.) so called Aida, Atlas and Odessa, are described. After 3 weeks of incubation at 28℃ ± 1℃, 70% of Aida microtubers variety, previously formed in the MT2 medium [MS/2 + 80 g·L^-1 Sucrose], germinated. The best germination rate for varieties Atlas (100%) and Odessa (66.66%) was obtained on microtubers previously formed in the medium MT2 [MS/2 + 100 g·L^-1 Sucrose]. The addition of hormones in the tuberization medium allowed optimizing the microtubers germination of the Aida variety unlike the other varieties. Indeed, for the Aida variety, the combination M5 [Kin 2.5 mg·L^-1 + Coum 0.025 mg·L^-1 + Sucrose 80 g·L^-1] increased the germination rate from 70% up to 93.33%. The best germination rate (90%), noticed with microtubers of Atlas variety, initially formed in M2 medium [Kin 1 mg·L^-1 + BAP 1 mg·L^-1 + Sucrose 100 g·L^-1], was lower than that one (100%) obtained on medium without hormones. For Odessa variety, the maximum germination rate (53.33%) of microtubers, from the medium M4 [Kin 2.5 mg·L^-1 + BAP 1 mg·L^-1 + Sucrose 100 g·L^-1], was also lower than that one (66.66%) observed in the medium without hormones. Aida and Atlas varieties thus offer a better germination rate than Odessa after their cold storage.

Keywords

Solanum tuberosum; Microcuttings; Microtubers; In Vitro Germination; Sucrose; Cytokinins; Coumarin

Conflicts of Interest

The authors declare no conflicts of interest.

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