Ionic Liquid-Based Ultrasound-Assisted Emulsification Microextraction Coupled with HPLC for Simultaneous Determination of Glucocorticoids and Sex Hormones in Cosmetics

Xiaoji Cao, Lingxiao Shen, Xuemin Ye, Feifei Zhang, Jiaoyu Chen, Weimin Mo

Research Center of Analysis and Measurement, Zhejiang University of Technology, Hangzhou, China.

DOI: 10.4236/gsc.2013.32A004                

Abstract

An effective and environmentally friendly method based on ionic liquid-based ultrasound-assisted emulsification microextraction(IL-USAEME) and high performance liquid chromatography (HPLC) has been developed for the determination of nine hormones including three glucocorticoids, one androgen and five progestogens in cosmetics. Several factors that affect the extraction efficiency, such as the kinds and volume of ionic liquid, salt concentration, ultrasonic power and time, and centrifugation time were investigated and optimized. Under the optimum extraction condition, the recoveries of analytes ranged from 85.97% to 108.84% except prednisolone (62.30%). The intraday and interday precision was below 2.51% and 3.30%, respectively.

Keywords

Ionic Liquid; Ultrasound-Assisted Emulsification Microextraction; Hormones; Cosmetics; High Performance Liquid Chromatography

Conflicts of Interest

The authors declare no conflicts of interest.

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