Evaluation of Physicochemical and Functional Properties of Protein Recovered Obtaining from Whitemouth Croaker (Micropogonias furnieri) Byproducts

Abstract

Proteins recovered were obtained from Whitemouth Croaker (Micropogonias furnieri) byproducts and their physico-chemical and functional properties were evaluated. The proximate composition, presented 8.64% ± 0.10% of moisture, 85.33% ± 0.12% of protein, 2.69% ± 0.09% of ashes and 2.16% ± 0.12% of lipids, in dry basis. The highest solubility was obtained in pH 11 (93.24%), the maximum water holding capacity was presented at pH 11 (25.71 mL H2O/gprotein), the oil holding capacity was 13.71 mL/gprotein and the digestibility in vitro was 91.32% ± 0.15%. The electro-phoretic profile was observed typical of the myofibrillar proteins, with the appearance of the heavy chain of myosin (220 kDa) and actin (50 kDa). The results show that the products of low commercial value of fish that are usually used for the production of animal feed or simply discarded, contributing to environmental pollution, may be used to produce products with a greater added value.

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F. Ferreira, B. Freire, J. Souza, W. Cortez-Vega and C. Prentice, "Evaluation of Physicochemical and Functional Properties of Protein Recovered Obtaining from Whitemouth Croaker (Micropogonias furnieri) Byproducts," Food and Nutrition Sciences, Vol. 4 No. 5, 2013, pp. 580-585. doi: 10.4236/fns.2013.45075.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] A. A. Goncalves and M. G. Passos, “Restructured Fish Product from White Croacker (Micropogonias furnieri) Mince Using Microbial Transglutaminase,” Brazilian Archives of Biology and Technology, Vol. 53, No. 4, 2010, pp. 987-995. doi:10.1590/S1516-89132010000400030
[2] H. Nolsoe and I. Undeland, “The Acid and Alkaline Solubilization Process for the Isolation of Muscle Proteins: State of the Art,” Food Bioprocess and Technology, Vol. 2, No. 1, 2009, pp. 1-27. doi:10.1007/s11947-008-0088-4
[3] I. R. Freitas, G. V. Gautério, D. G. Rios and C. Prentice, “Functionality of Protein Isolates from Argentine Anchovy (Engraulis anchoita) Residue Obtained Using pH Shift Processing,” Journal of Food Science and Engineering, Vol. 1, No. 5, 2011, pp. 374-378.
[4] A. K. Rai, H. C. Swapna, N. Bhaskar, P. M. Halami and N. M. Sachindra, “Effect of Fermentation Ensilaging on Recovery of Oil from Fresh Water Fish Viscera,” Enzyme and Microbial Technology, Vol. 46, No. 1, 2010, pp. 9-13. doi: 10.1016/2fj.enzmictec.2009.09.007
[5] E. S. G. Contreras, “Bioquímica de Pescados e Deriva dos,” FUNEP, Jaboticabal, 1994, pp. 315-330.
[6] Y. C. Chen and J. Jaczynski, “Protein Recovery from Rainbow Trout (Oncorhynchus mykiss) Processing By products via Isoelectric Solubilization/Precipitation and Its Gelation Properties as Affected by Functional Additives,” Journal of Agricultural and Food Chemistry, Vol. 55, No. 22, 2007, pp. 9079-9088. doi:10.1021/jf071992w
[7] L. Taskaya and J. Jaczynski, “Flocculation-Enhanced Protein Recovery from Fish Processing Byproducts by Isoeletric Solubilization/Precipitation,” Food Science and Technology, Vol. 42, No. 2, 2009, pp. 570-575.
[8] V. G. Martins, J. A. V. Costa, S. T. Silveira, A. Brandelli and C. H. Prentice, “Protein and Amino Acid Solubilization by Using Bacillus cereus, Bacillus velesensis, and Chryseobacterium sp. from Chemical,” Food and Bio process Tecnhology, Vol. 4, No. 1, 2011, pp. 116-123.
[9] Y. Thiansilakul, S. Benjakul and F. Shahidi, “Compositions, Functional Properties and Antioxidative Activity of Protein Hydrolysates Prepared from round Scad (Decapterus maruadsi),” Food Chemistry, Vol. 103, No. 4, 2007, pp. 1385-1394. doi:10.1016/j.foodchem.2006.10.055
[10] S. G. Chaud and V. C. Sgarbieri, “Propriedades Funcio nais (Tecnológicas) da Parede Celular de Leveduras da Fermenta??o Alcoólica e das Fracaes Glicana, Manana e Glicoproteína,” Ciência e Tecnologia de Alimentos, Vol. 26, No. 2, 2006, pp. 369-379. doi:10.1590/S0101-20612006000200020
[11] W. R. Cortez-Vega, G. G. Fonseca, V. A. Feisthera, T. F. Silva and C. Prentice, “Evaluation of Frankfurters Obtained from Croaker (Micropogonias furnieri) Surimi and Mechanically Deboned Chicken Meat Surimi-Like Material,” Journal of Food, Vol. 11, No. 1, 2013, pp. 27-36. doi:10.1080/19476337.2012.680199
[12] A.O.A.C, “Association of Official Analysis Chemists,” 16th Edition, Washington DC, 1995.
[13] M. Chalamaiah, G. N. Rao, D. G. Rao and T. Jyothirma yi, “Protein Hydrolysates from Meriga (Cirrhinus mrigala) Egg and Evaluation of Their Functional Properties,” Food Chemistry, Vol. 120, No. 3, 2010, pp. 652-657. doi:10.1016/j.foodchem.2009.10.057
[14] P. Tadpitchayangkoon, J. W. Park and J. Yongsawatdigul, “Conformational Changes and Dynamic Rheological Properties of Fish Sarcoplasmic Proteins Treated at Various pHs,” Food Chemistry, Vol. 121, No. 4, 2010, pp. 1046-1052. doi:10.1016/j.foodchem.2010.01.046
[15] O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Measurement with the Folin Phenol Reagent,” Journal of Biological Chemistry, Vol. 193, No. 1, 1951, pp. 265-275.
[16] J. M. Regenstein, C. A. Jauregui and R. Baker, “The Effect of pH, Polyphosphates and Different Salt on Water Retention Properties of Ground Trout Muscle,” Journal of Food Biochemistry, Vol. 8, No. 2, 1984, pp. 123-131. doi:10.1111/j.1745-4514.1984.tb00320.x
[17] M. M. Bradford, “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,” Analytical Bio chemistry, Vol. 72, No. 1-2, 1976, pp. 248-254. doi:10.1016/0003-2697(76)90527-3
[18] L. G. Fonkwe and R. K. Singh, “Protein Recovery from Mechanically Deboned Turkey Residue by Enzymic Hydrolysis,” Process Biochemistry, Vol. 31, No. 6, 1996, pp. 605-616. doi:10.1016/S0032-9592(95)00101-8
[19] V. Feddern, S. S. Pinto, K. A. Nogueira, E. B. Furlong and L. A. S. Soares, “Influência da Composicoo e da Fer mentacoo nas Propriedades Fisico-Quimicas e Nutrici onais de Multimisturas,” Brazilian Journal of Food Tech nology, Vol. 11, No. 2, 2008, pp. 128-133.
[20] U. K. Laemmli, “Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4,” Nature, Vol. 227, No. 1, 1970, pp. 680-685. doi:10.1038/227680a0
[21] G. S. Centenaro and M. S. Mellado, “Influência das Con centracoes de Enzima e de Substrato no Grau de Hidrólise e no Conteúdo Protéico de Hidrolisados Enzimáticos de Corvina (Micropogonias furnieri),” B. CEPPA, Vol. 26, No. 1, 2008, pp. 61-70.
[22] W. R. Cortez-Vega, G. G. Fonseca, V. A. Feisther, T. F. Silva and C. Prentice, “Evaluation of Frankfurters Obtained from Croaker (Micropogonias furnieri) Surimi and Mechanically Deboned Chicken Meat Surimi-Like Material,” CyTA-Journal of Food, Vol. 10, No. 1, 2012, pp. 190-199.
[23] V. G. Martins, J. A. V. Costa and C. Prentice, “Hidro lisado Proteíco de Pescado Obtido por vias Química e En zimática a Partir de Corvina (Micropogonias funieri),” Química Nova, Vol. 32, No. 1, 2009, pp. 61-66. doi:10.1590/S0100-40422009000100012
[24] S. Sathivel, P. Bechtel, W. P. Babbitt, I. I. Nesgulescu and K. Reppond, “Properties of Protein Powders from Arrowtooth Flounder (Atheresthes stomias) and Herring (Clupeaharengus) Byproducts,” Journal of Agricultural and Food Chemistry, Vol. 52, No. 16, 2004, pp. 5040-5046. doi:10.1021/jf0351422
[25] I. R, Freitas, “Otimiza??o do Processo de Obten??o de Isolados Proteicos Provenientes de Pescado de Baixo Valor Comercial,” Dissertation, University of Rio Grande, Rio Grande, 2011.
[26] S. Rawdkuen, S. Sai-ut, S. Khamsorn, M. Chaijan and S. Benjakul, “Biochemical and Gelling Properties of Tilapia Surimi and Protein Recovered Using Acid-Alkaline Pro cess,” Food Chemistry, Vol. 112, No. 1, 2009, pp. 112-119. doi:10.1016/j.foodchem.2008.05.047
[27] I. Batista, C. Pires and R. Nelhas, “Extraction of Sardine Proteins by Acidic and Alkaline Solubilisation,” Food Science and Technology International, Vol. 13, No. 3, 2007, pp. 189-194. doi:10.1177/1082013207079619
[28] L. M. B. Candido, A. K. Nogueira and V. C. Sgarbieri, “Propriedades Funcionais de Concentrados Proteicos de Pescado Preparados por Vários Métodos,” Brazilian Jour nal of Food Technology, Vol. 1, No. 1-2, 1998, pp. 77-89.
[29] H. O. Hultin and S. D. Kelleher, “Process for Isolating a Protein Composition from a Muscle Source and Protein Composition,” Advanced Protein Technologies Inc., Ro ckport, 1999.
[30] A. Fontana, G. S. Centenaro, S. C. Palezi and C. Prentice Hernández,” Obtencoo e Avaliacao de Concentrados Proteicos de Corvina (Micropogonias furnieri) Proces sados por Extracao Química,” Química Nova, Vol. 32, No. 9, 2009, pp. 2203-2299. doi:10.1590/S0100-40422009000900011
[31] C. S. Lin and J. F. Zayas, “Functionality of Defatted Corn Germ Proteins in a Model System: Fat Binding Capacity and Water Retention,” Journal of Food Science, Vol. 52, No. 5, 1987, pp. 1308-1311. doi:10.1111/j.1365-2621.1987.tb14070.x
[32] I. R. Freitas, G. V. Gautério, D. G. Rios and C. Prentice, “Functionality of Protein Isolates from Argentine Anchovy (Engraulis anchoita) Residue Obtained Using pH Shift Processing,” Journal of Food Science and Engineering, Vol. 1, No. 5, 2011, pp. 374-378.
[33] S. Sathivel and P. Bechtel, “Properties of Soluble Protein Powders from Alaska Pollock (Theragra chalcogram ma),” International Journal of Food Science and Technology, Vol. 41, No. 5, 2006, pp. 520-529. doi:10.1111/j.1365-2621.2005.01101.x
[34] L. I. A. Castro, C. M. Vila Real and C. I. S. Pires, “Qui noa (Chenopodium quinoa willd): Digestibilidade in Vitro, Desenvolvimento e Análise Sensorial de Prepara??es Destinadas a Pacientes Celíacos,” Alimentos e Nutri??o, Vol. 18, No. 4, 2007, pp. 413-419.
[35] P. G. Costa, A. Fontana, I. Veiga and C. Prentice, “Carac teriza??o Funcional e Nutricional de um Isolado Protéico Obtido a Partir de Resíduos de Camar?o Rosa (Farfan tepenaeus paulensis),” Alimenta??o e Nutri??o, Vol. 18, No. 1, 2007, pp. 7-18.
[36] P. Tongnuanchan, S. Benjakul and T. Prodpran, “Roles of Lipid Oxidation and pH on Properties and Yellow Discolouration during Storage of ?lm from Red Tilapia (Oreochromis niloticus) Muscle Protein,” Food Hydro colloids, Vol. 25, No. 3, 2011, pp. 426-433. doi:10.1016/j.foodhyd.2010.07.013
[37] E. S. Monterrey-Quintero and P. J. A. Sobral, “Preparo e Caracteriza??o de Proteínas Miofibrilares de Tilápia-do Nilo Para Elabora??o de Biofilmes,” Pesquisa Agrope cuária Brasileira, Vol. 35, No. 1, 2000, pp. 179-189. doi:10.1590/S0100-204X2000000100020
[38] B. Cuq, N. Gontard, J. Cuq and S. Guilbert, “Functional Properties of Myofibrilar Protein-Based Biopackaging as Affected by Film Thickness,” Journal of Food Science, Vol. 61, No. 3, 1996, pp. 580-584. doi:10.1111/j.1365-2621.1996.tb13163.x

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