The Effects of a Primary Nutritional Deficiency (Vitamin B study)

Hugh D. Riordan, Nina Mikirova, Paul R. Taylor, Cindy A. Feldkamp, Joseph J. Casciari

The Riordan Clinic Research Institute; Riordan Clinic; Wichita, Kansas, USA.

DOI: 10.4236/fns.2012.39163                

Abstract

It has long been understood there is a link between a nutrient deficiency and visually measurable health. In separate studies, Sprague Dawley or Fischer 344 rats were fed diets lacking 100% of one B-Vitamin until a single mortality occurred. Vitamins studied were thiamin, riboflavin, niacin, pyridoxine, pantothenic acid, cobalamin and folate. Body mass, food consumption and images were recorded at weekly intervals. Rats were euthanized and liver, kidney, heart and brain samples for histological analysis were prepared. Liver and brain tissues were chosen as markers because of the high need of that organ for B vitamins and the likelihood of secondary lesion development. Correlation of deficiency symptoms with one missing nutrient was explored.

Keywords

Essential Nutrients; B Vitamins; Deficiency Symptoms; Animal Study

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	C. Funk, “The Vitamins,” Williams and Wilkins, Baltimore, 1922.
[2]	V. Tanphaichitr , “Thiamin,” In: M. E. Shils, J. A. Olsen and M. Shike, Eds., Modern Nutrition in Health and Disease, Williams and Wilkins, Baltimore, 1999, pp. 381-389.
[3]	G. Rindi, “Thiamin,” In: E. E. Ziegler and L. J. Filer, Eds., Present Knowledge in Nutrition, ILSI Press, Washington DC, 1996, pp. 160-166.
[4]	T. Brody, “Nutritional Biochemistry,” 2nd Edition, Academic Press, San Diego, 1999.
[5]	Micronutrient Information Center, Linus Pauling Institute at Oregon State University, 2012. http://lpi.oregonstate.edu/infocenter/vitamins/
[6]	D. Cervantes-Laurean, N. G. McElvaney and J. Moss, “Niacin,” In: M. Shils, J. A. Olson, M. Shike and A. C. Ross, Eds., Modern Nutrition in Health and Disease, Williams and Wilkins, Baltimore, 1999, pp. 401-411.
[7]	P. C. Fry, H. M. Fox and H. G. Tao, “Metabolic Response to a Pantothenic Acid Deficient Diet in Humans,” Journal of Nutritional Science and Vitaminology, Vol. 22, No. 4, 1976, pp. 339-346. HUdoi:10.3177/jnsv.22.339U
[8]	D. B. McCormick, “Vitamin B6,” In: B. A. Bowman and R. M. Russell, Eds., Present Knowledge in Nutrition, Vol. 1, International Life Sciences Institute, Washington DC, 2006, pp. 269-277.
[9]	J. E. Leklem, “Vitamin B6,” In: L. Machlin, Ed., Handbook of Vitamins, Marcel Decker Inc., New York, 1991, pp. 341-378.
[10]	S. Dakshinamurti and K. Dakshinamurti, “Vitamin B6,” In: J. Zempleni, R. B. Rucker, D. B. McCormick and J. W. Suttie, Eds., Handbook of Vitamins, CRC Press (Taylor & Fracis Group), New York, 2007, pp. 315-359.
[11]	J. E. Leklem, “Vitamin B6,” In: M. Shils, J. A. Olson , M. Shike and A.C. Ross, Eds., Modern Nutrition in Health and Disease, Williams & Wilkins, Baltimore, 1999, pp. 413-422.
[12]	A. D. Mackey, S. R. Davis and J. F. Gregory III, “Vitamin B6,” In: M. E. Shils, M. Shike, A.C. Ross, B. Caballero and R. J. Cousins, Eds., Modern Nutrition in Health and Disease, Williams and Wilkins, Philadelphia, 2006, pp. 452-461.
[13]	N. J. Wald, M. R. Law, J. K. Morris and D. S. Wald, “Quantifying the Effect of Folic Acid,” Lancet, Vol. 358, No. 9298, 2001, pp. 2069-2073. HUdoi:10.1016/S0140-6736(01)07104-5U
[14]	L. B. Bailey and J. F. Gregory III, “Polymorphisms of Methylenetetrahydrofolate Reductase and Other Enzymes: Metabolic Significance, Risks and Impact on Folate Requirement,” Journal of Nutrition, Vol. 129. No. 5, 1999, pp. 919-922.
[15]	H. McNulty, G. J. Cuskelly and M. Ward, “Response of Red Blood Cell Folate to Intervention: Implications for Folate Recommendations for the Prevention of Neural Tube Defects,” American Journal of Clinical Nutrition, Vol. 7, No. 5, 2000, pp. 1308S-1311S.
[16]	S. W. Choi and J. B. Mason, “Folate and Carcinogenesis: An Integrated Scheme,” Journal of Nutrition, Vol. 130. No. 2, 2000, pp. 129-132.
[17]	B. Shane, “Folic Acid, Vitamin B12, and Vitamin B6,” In: M. Stipanuk, Ed., Biochemical and Physiological Aspects of Human Nutrition, W.B. Saunders Co., Philadelphia, 2000, pp. 483-518.
[18]	Food and Nutrition Board, Institute of Medicine, “Vitamin B12. Dietary Reference Intakes: Thiamin, Riboflavin, Niacin, Vitamin B6, Vitamin B12, Pantothenic Acid, Biotin, and Choline,” National Academy Press, Washington DC, 1998, pp. 306-356.
[19]	V. Herbert, “Vitamin B12,” In: E. E. Ziegler and L. J. Filer, Eds., Present Knowledge in Nutrition, 7th Edition, ILSI Press, Washington DC, 1996, pp. 191-205.
[20]	P. G. Reeves, F. H. Nielsen and G. C. Fahey Jr., “AIN-93 Purified Diets for Laboratory Rodents,” Journal of Nutrition, Vol. 123, No. 11, 1993, pp. 1939-1951.
[21]	A. Odynets, “Beddings of Laboratory Animals: Criteria of Biological Evaluation,” Lab. Zyhvotnye, Vol. 1, No. 3, 1991, pp. 70-76.
[22]	A. J. Clifford, D. S. Wilson and N. D. Bills, “Repletion of Folate-Depleted Rats with an Amino Acid-Based Diet Supplemented with Folic Acid,” Journal of Nutrition, Vol. 119, No. 12, 1989, pp. 1956-1961.
[23]	D. Nakae , H. Yoshiji, Y. Mizumoto , K. Horiguchi , K. Shiraiwa , K. Tamura, A. Denda and Y. Konishi, “High Incidence of Hepatocellular Carcinoma Induced by a Choline Deficient L-Amino Acid Defined Diet in Rats,” Cancer Research, Vol. 52, No. 18, 1992, pp. 5042-5045.
[24]	D. Nakae, H. Yoshiji, H. Maruyama, T. Kinugasa, A. Denda and Y. Konishi, “Production of Both 8-Hydroxydeoxyguanosine in Liver DNA and y-Glutamyltransferase-posistive Hepatocellular Lesions in Rats Given a Choline-deficient, L-Amino Acid-defined Diet,” Journal of Cancer Research, Vol. 81, 1990, pp. 1081-1084.
[25]	N. Sawada, L. Poirier, S. Moran, Y. H. Xu and H. C. Pitot, “The Effect of Choline and Methionine Deficiencies on the Number and Volume Percentage of Altered Hepatic Foci in the Presence or Absence of Diethylnitrosamine Initiation in Rat Liver,” Carcinogenesis (Lond.), Vol. 11, No. 2, 1990, pp. 273-281. HUdoi:10.1093/carcin/11.2.273U
[26]	J. C. Gonder and K. Laber, “A Renewed Look at Laboratory Rodent Housing and Management,” Institute of Laboratory Animal Resources Journal, Vol. 48, No. 1, 2007, pp. 29-36.
[27]	R. H. Weichbrod, C. F. Cisar, J. G. Miller, R. C. Simmonds, A. P. Alvares and T. H. Ueng, “Effects of Cage Beddings on Microsomal Oxidative Enzymes in Rat Liver,” Laboratory Animal Science, Vol. 38, No. 3, 1988, pp. 296-298.
[28]	M. Maeno, Y. Morimoto, T. Hayakawa, Y. Suzuki and H. Tsuge, “Feeding Experiments of Pyridoxine Derivatives as Vitamin B6,” International Journal for Vitamin and Nutrition Research, 1997, Vol. 67, No. 6, pp. 444-449.
[29]	K. Dakshinamurti, S. K. Sarma and D. Bonke, “Influence of B Vitamins on Binding Properties of Serotonin Receptors in the CNS of Rats,” Klinische Wochenschrift, Vol. 68, No. 2, 1990, pp. 142-145. HUdoi:10.1007/BF01646863U
[30]	B. C. Richardson, “Methylation in the Regulation of Cell Function: Autoimmunity, Aging, and Cancer,” Journal of Nutrition, Vol. 132, No. 8, 2002, pp. 2401S-2405S.
[31]	J. F. Costello and C. Plass, “Methylation Matters,” Journal of Medical Genetics, Vol. 38, No. 5, 2001, pp. 285-303. HUdoi:10.1136/jmg.38.5.285U
[32]	C. Amoreira, W. Hindermann and C. Grunau, “An Improved Version of the DNA Methylation Database (MethDB),” Nucleic Acids Research, Vol. 31, No. 1, 2003, pp. 75-77. HUdoi:10.1093/nar/gkg093U